中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 西部白眉长臂猿支气管败血波氏杆菌的分离鉴定及其耐药基因检测 西北农业学报 2020,29 (5) 668-674
Position: Home > Articles > Isolation and Identification of Bordetella bronchiseptica from Western Hylobates hoolock and Detection of Resistance Gene Acta Agriculturae Boreali-occidentalis Sinica 2020,29 (5) 668-674

西部白眉长臂猿支气管败血波氏杆菌的分离鉴定及其耐药基因检测

作  者：
姜睿姣;张鹏飞;王印;姚学萍;杨泽晓;罗燕
单  位：
关键词：
白眉长臂猿;支气管败血波氏杆菌;分离鉴定;超广谱β-内酰胺酶
摘  要：
为探究某动物园西部白眉长臂猿死亡的可能原因,无菌采集病死白眉长臂猿肺脏组织进行细菌分离鉴定。通过分离纯化、培养特性观察、革兰染色镜检、生化试验以及16S rDNA克隆测定对分离菌株进行鉴定;进一步研究分离株的致病性、耐药性,并进行超广谱β-内酰胺酶耐药基因检测及测序分析。结果表明,分离得到1株革兰阴性小杆菌,命名为J181121,该分离菌的培养及生化特性符合支气管败血波氏杆菌,其16S rDNA基因序列与Genbank中支气管败血波氏杆菌(登录号:CP018761.1)的同源性为99%,对小鼠致病力强。药敏试验结果显示,分离株对β-内酰胺类抗生素耐药,PCR检测到超广谱β-内酰胺酶耐药基因bla-SHV。
译  名：
Isolation and Identification of Bordetella bronchiseptica from Western Hylobates hoolock and Detection of Resistance Gene
作  者：
JIANG Ruijiao;ZHANG Pengfei;WANG Yin;YAO Xueping;YANG Zexiao;LUO Yan;College of Veterinary Medicine, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;
关键词：
Hylobates hoolock;;Bordetella bronchiseptica;;Isolation and identification;;Extended-spectrumβ-lactamases
摘  要：
In order to explore the possible death cause of the Hylobates hoolock in a zoo, the bacteria was isolated and identified from the lungs. Isolation and purification, culture characteristics observation, gram staining microscopy, biochemical test and 16 S rDNA cloning were conducted to identify the isolated strains. The pathogenicity and drug resistance of the isolates were further studied, and the ultrabroad spectrum beta-lactamase resistance genes were tested and sequenced. The result showed that a strain of gram-negative bacillus was successfully isolated, which was named J181121. The isolate was tested for pathogenicity and drug resistance, and further extended-spectrum β-lactamase resistance genes were detected and sequenced. The culture and biochemical characteristics of J181121 were consistent with Bordetella bronchiseptica, and the 16 S rDNA gene sequence of this isolate showed 99% homology with B. bronchiseptica(accession number: CP018761.1) in genbank. And it was highly pathogenic to mice. The drug sensitivity test showed that the isolate was resistant to β-lactam antibiotics, and the extended-spectrum β-lactamase gene bla-SHV could be amplified by PCR.

相似文章

PDF下载 PDF预览
导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46