摘 要：
杂色山雀是一种分布区域狭窄的小型森林洞巢鸟类,在生态学上属衰落物种。杂色山雀是单态性鸟,仅从外部形态辨别雌雄非常困难。本研究采用非伤害性取样法以Chelex-100为介质快速提取DNA,利用5只已知性别的杂色山雀,对CHD基因性别鉴定特异性引物P2/P8和2550F/2718R的有效性进行验证,比较两对引物PCR的反应结果,筛选出用于杂色山雀性别鉴定的有效引物P2/P8,并摸索该引物的最佳PCR反应条件和反应体系,寻找到一种快捷方便、采样量少、鉴定精度高的性别鉴定的分子方法,为日后统计杂色山雀的种群性比现状提供基本技术方法。
译 名：
Studies on a molecular method of sex identification of Parus varius
作 者：
HUO Ya-peng,CAO Jun,ZHANG Yu-ting,WAN Dong-mei (College of Life Science,Liaoning University,Shenyang110036,China)
关键词：
Parus varius;sex identification;PCR
摘 要：
Parus varius is a kind of small cavity-nest forest birds with obvious narrow distribution,which is one of the endangered wild species.Parus varius belongs to monomorphic birds.It is difficult to identify their sex from exterior morphology.In this pa- per,DNA was extracted by Chelex-100 for the further tests based on nondestructive samples.In the experiment,the validity of two pairs of primers,P2/P8 and 2550F/2718R of CHD gene,was estimated by 5 individuals(3 males and 2 females)that had been sexed previously on the basis of incubation.The results indicated that the pair of primers P2/P8 was effective,and the perfect reac- tion systems of PCR were found out.This molecular method has shown its advantages as a safe,convenient and accurate method for bird sex identification,which would be very useful for the future study of sex ratio of Parus varius of all subspecies.
