Preventive Effect of Procyanidins from Lotus Seedpod on Hippocampal Neurons Injury Induced by Extremely Low Frequency Electromagnetic Field

ZHANG Hai-hui1,WU Yan1,ZHANG Rui1,ZHANG Shuang1,DUAN Yu-qing1,,SUN Gui-bo2,SUN Xiao-bo2(1.School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;2.Institute of Medicinal Plants,Chinese Academy of Medical Sciences,Beijing 100193,China)

extremely low frequency electromagnetic field;hippocampal neuron;procyanidins from lotus seedpod(LSPC);reactive oxygen species(ROS)

To investigate the preventive effect of procyanidins from lotus seedpod(LSPC) on hippocampal neuron injury induced by extremely low frequency electromagnetic fields(ELF-EMF),primary cultured hippocampal neurons from rats were pretreated with LSPC before exposure to ELF-EMF.Survival rates,the morphology of neurons and the level of reactive oxygen species(ROS) were measured.The survival rates of hippocampal neurons pretreated with LSPC(2.5,5 μg/mL and 10 μg/mL) were 81.23%,90.18% and 93.45%,respectively,which were significantly higher than those from the model group(66.88%,P < 0.05).Electromagnetic radiation increased the ROS level in neurons(the fluorescence intensity was(228.19 ± 16.28),while pretreatment with 2.5,5,10 μg/mL of LSPC reduced ROS level obviously(P < 0.05).All these studies indicated pretreatment with LSPC could significantly block hippocampal neuron injury induced by extremely low frequency electromagnetic field.