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当前位置: 首页 > 文章 > 环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析 中国畜牧兽医 2016,43 (7) 1818-1825
Position: Home > Articles > Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs China Animal Husbandry & Veterinary Medicine 2016,43 (7) 1818-1825

环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析

作  者：
李俊;许钟峯;张海航;尚月丽;孔祥峰;石德顺;刘庆友
单  位：
中国科学院亚热带农业生态研究所;广西大学
关键词：
环江香猪;黏附素13基因;单核苷酸多态性;单倍型;仔猪腹泻
摘  要：
试验旨在对环江香猪仔猪腹泻抗性/易感的黏附素13基因(MUC13)单核苷酸多态性(single nucleotide polymorphism,SNP)/单倍型与仔猪腹泻的相关性进行分析。试验采用高分辨率熔解曲线(high resolution melting,HRM)技术对93头环江香猪腹泻仔猪和95头健康对照仔猪进行基因分型,结合DNA测序对HRM检测结果进行验证,并通过SHEsis在线平台确定其单倍型。结果显示,环江香猪MUC13基因的G224A位等位基因分布频率在腹泻组和对照组间差异显著(P<0.05),GG、GA和GG+GA基因型分别与AA基因型相比差异均显著(P<0.05)。A112G、T170C和G164C位点各基因型频率和等位基因频率在腹泻组和对照组之间差异均不显著(P>0.05),腹泻组与对照组E位点均为野生纯合基因型。单倍型Ⅹ(GTAC)在腹泻组的分布极显著高于对照组(P<0.01)。因此,MUC13基因G224A位点和单倍型Ⅹ(GTAC)与环江香猪仔猪断奶前腹泻相关。
译  名：
Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs
作  者：
LI Jun;XU Zhong-feng;ZHANG Hai-hang;SHANG Yue-li;KONG Xiang-feng;SHI De-shun;LIU Qing-you;State Key Laboratory of Tropical Biological Resources Protection and Utilization of Nanning,Guangxi University;Institute of Subtropical Agriculture Chinese Academy of Sciences;
关键词：
Huanjiang miniature pigs;;MUC13gene;;SNP;;haplotypes;;piglet diarrhea
摘  要：
The purpose of the study was to analyze the association between MUC13 gene polymorphisms and piglet diarrhea in Huanjiang miniature pigs.93 diarrhea piglets and 95 healthy controls were included in the study.The MUC13 gene polymorphisms were determined using high resolution melting(HRM),and the results of HRM were verified by direct sequencing.Mutations were compared between diarrhea piglets and healthy controls.SHEsis online platform was used to calculate the haplotypes.There were statistically significant association on G224 Asite showed significant difference between diarrhea piglets and healthy controls by allele frequencies(P<0.05),and there were also statistically significant differences between the two groups by genotypes among GG,GA,GG+GA and AA(P<0.05).But no statistically significant association was found between polymorphisms of A112 G,T170Cand G164 Csites and piglet diarrhea risk(P>0.05).However,haplotypeⅩ(GTAC)in the distribution of diarrhea group was extremely significantlyhigher(P<0.01).The present data revealed that MUC13 gene G224Asite and the haplotypesⅩ(GTAC)were associated with reduce risk of piglet diarrhea.

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