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当前位置: 首页 > 文章 > 辽宁省豇豆轮纹病病原菌鉴定及其生物学特性 中国蔬菜 2013,1 (18) 88-95
Position: Home > Articles > Identification of Pathogen of Cowpea Ring Spot and Its Biological Characteristics by Liaoning Province China Vegetables 2013,1 (18) 88-95

辽宁省豇豆轮纹病病原菌鉴定及其生物学特性

作  者：
刘志恒;李健冰;安心;唐爽爽;郑川;王世维
单  位：
沈阳农业大学植物保护学院
关键词：
辽宁省;豇豆轮纹病;多主棒孢霉;生物学特性
摘  要：
对辽宁省新发现的病害——豇豆轮纹病的发病症状进行了描述,并对其病原菌进行了鉴定,对病菌生物学特性进行了初步测定。结果表明,通过柯赫氏法则证病试验,经形态学及分子生物学技术鉴定,并与基因库中Corynespora cassiicola(Berk and Curt.)序列进行比对,相似性达99%,鉴定豇豆轮纹病的致病菌为多主棒孢霉Corynespora cassiicola(Berk and Curt.)。生物学特性测定结果表明,病原菌菌丝在豇豆叶煎汁培养基上生长最快,碳源和氮源以甘露醇和牛肉浸膏最佳,最适温度为28℃,最适pH为8～10;分生孢子产生的最佳培养基为豇豆种子煎汁培养基,最佳碳源为麦芽糖,最佳氮源为牛肉浸膏,最适温度为28℃,最适pH值为9,光暗交替培养利于孢子的产生;分生孢子萌发的最适温度为28℃,最适pH值为7～10;菌丝致死温度为65℃,10min;分生孢子致死温度为60℃,10min。
译  名：
Identification of Pathogen of Cowpea Ring Spot and Its Biological Characteristics by Liaoning Province
作  者：
LIU Zhi-heng,LI Jian-bing,AN Xin,TANG Shuang-shuang,ZHENG Chuan,WANG Shi-wei (Plant Protection College,Shenyang Agricultural University,Shenyang 110866,Liaoning,China)
关键词：
Liaoning Province;Cowpea ring spot;Corynespora cassiicola;Biological characteristics
摘  要：
The newly discovered cowpea disease in Liaoning Province—cowpea ring spot's paroxysm symptom was described and its pathogen was identified in this paper.The pathogen biological characteristics was also preliminary determined. The results showed that through experiment of Koch's rule disease test,identification by morphology and molecular biology technology,and comparison with sequence of Corynespora cassiicola(Berk and Curt.)in GenBank,we found that the homology was up to 99%.Therefore,the pathogen of cowpea ring spot was C. cassiicola(Berk and Curt). The results of biological characteristics determination showed that cowpea leaf juice was the best medium for mycelia growth.Mannitol and beef extract were the best carbon and nitrogen sources.The optimum temperature was 28 ℃,and the optimal pH value was 8-10.The most beneficial medium for conidial production was cowpea seed juice. The best carbon source was maltose and the best nitrogen source was beef extract. The optimum temperature was 28 ℃,and the optimal pH value was 9.The optimum temperature for conidial germination was 28 ℃ and the optimal pH value was 7-10.The lethal temperature for spore was 60 ℃ for 10 minutes,and that for mycelia was 65 ℃ for 10 minutes.

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