中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 外源Wnt3a对体外培养奶牛乳腺上皮细胞数量及Frizzled 1受体和β-catenin表达的影响 畜牧兽医学报 2014,45 (8) 1282-1287
Position: Home > Articles > Effect of Exogenous Wnt3a on Mammary Cell Number in Cow and the Expression of Frizzled 1 Receptor and β-catenin Chinese Journal of Animal and Veterinary Sciences 2014,45 (8) 1282-1287

外源Wnt3a对体外培养奶牛乳腺上皮细胞数量及Frizzled 1受体和β-catenin表达的影响

作  者：
崔亚利;孙海云;李建国;高艳霞;赵艳姣;李三吓
单  位：
河北农业大学动物科技学院;河北农业大学动物医学院
关键词：
奶牛;乳腺上皮细胞;Wnt3a;β-catenin;Frizzled 1受体
摘  要：
旨在研究外源性重组W:nt3a蛋白对奶牛乳腺组织中上皮细胞数量及其Wnt信号通路中Frizzled 1受体和β-catenin mRNA表达的影响。采用组织块法培养奶牛乳腺上皮细胞,Western-blot方法对培养液中的β-酪蛋白进行鉴定。待组织块贴壁后,向培养液中分别加入0、10、25和50 ng·mL~(-1)的Wnt3a蛋白,并收集培养至10 d的细胞培养液和细胞。荧光定量PCR检测细胞中Frizzled 1受体和β-catenin mRNA的表达量。结果表明:50ng·mL~(-1)的Wnt3a蛋白对增加乳腺上皮细胞数量的作用较明显,Frizzled 1受体mRNA表达量显著增加,β-cate—nin mRNA表达量与对照组间没有差异。结果提示,外源Wnt 3a能够影响体外培养奶牛乳腺上皮细胞Frizzled 1受体表达量,增加乳腺上皮细胞的数量,提示Wnt信号通路可能参与乳腺组织生长发育的调控。
译  名：
Effect of Exogenous Wnt3a on Mammary Cell Number in Cow and the Expression of Frizzled 1 Receptor and β-catenin
作  者：
CUI Ya-li;SUN Hai-yun;LI Jian-guo;GAO Yan-xia;ZHAO Yan-jiao;LI San-xia;College of Veterinary Medicine,Agricultural University of Hebei;College of Animal Science and Technology,Agricultural University of Hebei;
关键词：
bovine;;mammary epithelial cells;;Wnt3a;;β-catenin;;Frizzled 1 receptor
摘  要：
This experiment was aimed at studying the effects of exogenous Wnt3 a on bovine mammary epithelial cell(BMEC) number and the expression of Frizzled 1 receptor and β-catenin.BMEC was cultured by adherent tissue culture.β-casein of culture medium was detected by Western-blot.BMEC was cultured in medium with 0,10,25 and 50 ng ? mL~(-1) Wnt3 a separately,and then the BMEC and culture medium at 10 days were collected.The expression of Frizzled 1 receptor and β-catenin mRNA was checked by real time RT-PCR.The results showed that cell numbers in 50 ng ? mL~(-1) Wnt3 a group were significantly higher than those of control group.The expression of Frizzled 1 receptor significantly increased,and there was not much difference in β-catenin expression between control group and Wnt3 a group.It was concluded that Wnt3 a exerts effects on the expression of Frizzled 1 receptor,moreover,promotes the number of BMEC.Current evidence suggested that Wnt signal played roles in the regulation of mammary development.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46