中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 采用高效液相色谱-串联质谱法检测芍药中褪黑素含量 分子植物育种 2017,15 (4) 1527-1534
Position: Home > Articles > Analysis of Melatonin in Herbaceous Peony(Paeonia lactiflora Pall.) by High Performance Liquid Chromatography-tandem Mass Spectrometry Molecular Plant Breeding 2017,15 (4) 1527-1534

采用高效液相色谱-串联质谱法检测芍药中褪黑素含量

作  者：
刘定;赵大球;陆春良;李志远;陶俊
单  位：
扬州大学动物科学与技术学院扬州大学园艺与植物保护学院扬州大学测试中心;扬州大学测试中心;扬州大学动物科学与技术学院扬州大学园艺与植物保护学院;扬州大学园艺与植物保护学院
关键词：
高效液相色谱-串联质谱;芍药;褪黑素;多反应监测
摘  要：
建立高效液相色谱-串联质谱(HPLC-MS/MS)专一性测定芍药褪黑素含量的检测方法,采用超声波甲醇提取芍药样品中的褪黑素。色谱条件:色谱柱为Thermo a Q-C18(2.1×50 mm,2.6μm),流动相为乙腈和0.5%甲酸水溶液,梯度洗脱,柱温30℃,进样量5μL,流速0.4 mL/min。质谱条件:电喷雾正离子模式(ESI+)电离,多反应监测模式(MRM)检测,检测离子对为m/z 233.2→m/z 174.2、233.2→m/z 159.2。在25 min内褪黑素得到很好的分离并被检测,褪黑素在2~200 ng/mL范围内呈良好的线性关系、精密度、重复性和稳定性的RSD均小于10%,回收率在64.87%~74.59%之间,平均回收率70.06%。本方法灵敏度高、专一性强、结果可靠准确,可为测定芍药不同组织中褪黑素含量提供一种高效精准的检测方法。
译  名：
Analysis of Melatonin in Herbaceous Peony(Paeonia lactiflora Pall.) by High Performance Liquid Chromatography-tandem Mass Spectrometry
作  者：
Liu Ding;Zhao Daqiu;Lu Chunliang;Li Zhiyuan;Tao Jun;College of Animal Science and Technology,Yangzhou University;College of Horticulture and Plant Protection,Yangzhou University;Testing and Analysis Center,Yangzhou University;
关键词：
HPLC-MS/MS chromatography;;Paeonia lactiflora Pall.;;Melatonin;;Multiple reaction monitoring
摘  要：
To establish a HPLC-MS/MS method to determine the contents of melatonin with multi-reactions monitoring(MRM) technology in different organs of Paeonia lactiflora Pall.and melatonin was extracted from samples with methanol under the assistance of ultrasonic.The HPLC condition was as follows:separation column:Thermo a Q-C18 column(2.1×50 mm,2.6 μm);mobile phase,acetonitrile-0.5% methanoic acid solution with gradient elution;injection volume:5 μL;flow rate:0.4 mL/min;column temperature:30℃.Mass spectrometric conditions:the electrospray was operated under the positive ionization mode and the drug was identified by multiple reaction monitoring(MRM) mode.The ion of monitor was at m/z 233.2→m/z 174.2 and m/z233.2 →m/z 159.2.Melatonin was well separated and detected in 25 minutes and the melatonin standards represented a good linear relationship with the range of 2~200 ng/mL.The recovery varied from 64.87% to74.59% and the average recovery was 70.06%.The relative standard deviration was lower than 10%.Conclusion:The method has high sensitivity,better specificity and accurate results which can be a high-efficiency and accurate method for the determination of melatonin in Paeonia lactiflora Pall..

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46