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当前位置: 首页 > 文章 > 小杆线虫Rhabditis(Oscheius)sp.生物学特性测定 安徽农业科学 2010,38 (1) 222-223+243
Position: Home > Articles > Determination on Biological Characteristics of Nematode Rhabditis(Oscheius) sp. Journal of Anhui Agricultural Sciences 2010,38 (1) 222-223+243

小杆线虫Rhabditis(Oscheius)sp.生物学特性测定

作  者：
张丽娟;刘奇志;王玉柱;杜小康;孙浩元;Zdeneck Mrá(c)ek
单  位：
捷克科学院昆虫研究所生物学中心;中国农业大学昆虫系昆虫与线虫学实验室;北京市农林科学院林业果树研究所
关键词：
小杆线虫;生物学;侵染力;耐热性
摘  要：
[目的]对从内蒙古自治区呼伦贝尔盟海拉尔市苜蓿根际土壤中分离出的小杆线虫[Rhabditis(Oscheius)sp.]的生物学特性进行测定。[方法]以大蜡螟(Galleria mellonella)和黄粉甲(Tenebrio molitor)老熟幼虫为寄主,测定小杆线虫的侵染力;以水浴方法测定小杆线虫的耐热能力。[结果]小杆线虫在10、20和40 IJs/虫的剂量下侵染力较低;但在80、160、320和640 IJs/虫的剂量下侵染力较高,大蜡螟96 h、黄粉甲120 h的校正死亡率均达90%以上;在80 IJs/虫的剂量下侵染力最高,侵染120 h,寄主的校正死亡率达100%。浓度1 000~5 000 IJ/ml的小杆线虫在36℃条件下水浴2 d,有25%左右的个体存活,水浴6 d,仍有3%~9%的个体存活,至第9天,线虫个体全部死亡;在38℃下水浴6 h、40℃下水浴2 h,线虫个体全部死亡。[结论]小杆线虫具有较强的侵染力和耐热能力,具有开发应用潜能。
译  名：
Determination on Biological Characteristics of Nematode Rhabditis(Oscheius) sp.
作  者：
ZHANG Li-juan et al(Key Laboratory for Biological Control of Ministry of Agriculture,Laboratory of Entomology and Nematology,Department of Entomology,China Agricultural University,Beijing 100193)
关键词：
Rhabditis(Oscheius) sp.;Biology;Infective ability;Heat tolerance
摘  要：
[Objective] The study aimed to determine the biological characteristics of Rhabditis(Oscheius) sp.isolated from rhizosphere soil of alfalfa in Hailar City,Hulunbeir League,Inner Mongolia Autonomous.[Method] With mature larvae of Galleria mellonella and Tenebrio molitor as the host,the infective ability of nematode was determined,and its heat tolerance ability was measured by using water bath method.[Result] The infective ability of nematode was lower at the dosages of 10,20 and 40 IJs/larva,but higher at the dosages of 80,160,320 and 640 IJs/larva.The corrected mortality(CM) of G.mellonella at 96 h and T.molitor at 120 h were both above 90%.The nematode infective ability was highest at the dosage of 80 IJs/larva.The hosts'CM reached to 100% after 120 h infection.About 25% individuals could survive in 36 ℃ water bath for 2 d with the densities of 1 000-5 000 IJ/ml,up to 6 d,there were still 3%-9% individuals survived,until 9 d,the nematodes died totally.A hundred percent of the nematode individuals died in 38 ℃ water bath after 6 h,in 40 ℃ water bath after 2 h.[Conclusion] Rhabditis(O.) sp.has the potential of development and application in higher host infection capacity and heat tolerance.

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