作 者：
薛运波;李兴安;葛凤晨;蒋滢;历延芳;闫德斌;常志光
关键词：
长白山中华蜜蜂;RAPD;克隆;分子标记;CBS-70(0bp)DNA片段
摘 要：
本文通过分析12个长白山中蜂(长白山地方采样点中华蜜蜂)基因组多态性分布规律,以寻找其基因组特异性分子标记为主要目的;以RAPD-PCR技术选择性扩增基因组RAPD位点分子,以克隆有意义的分子标记,DNA酶Ⅰ将克隆的分子标记酶学合成为探针并进行Southern杂交等为主要方法;结果发现1005号蜂等10个基因组分子标记多态性图谱同时显示700(bp)DNA片段,而1083号蜂等2个基因组分子标记多态性图谱仅为弱显示或未显示700(bp)DNA片段;这一结果提示:700(bp)DNA片段可能是长白山中蜂基因组有意义的分子标记,即保守的同源序列,可以充当长白山中蜂基因组的特异性分子标记。
译 名：
The Study on cloning homogeneous CBS-700(bp) DNA fragment and Southern hybridization of Different Groups from Local Changbaishan Apis Cerana ceranas
作 者：
XUE Yun-Bo1, LI Xing-An1, GE Feng-Chen1, JIANG Ying2, LI Yan-Fang1, YAN De-Bing1,CHANG Zhi-Guang1(1Institute of Scientific Apiculture of Jilin Province, Jilin 132108; 2 Medical School of Suzhou University, Suzhou 215006 )
关键词：
Changbaishan Apis cerana cerana , RAPD, clone, Molecular biomarks , CBS-700 band of DNA fragment
摘 要：
[OBJECTIVE]The article was about sorting specific molecular biomarker by analyzing polymorphism map of genome of local Changbaishan Apis cerana ceranas;[METHOD]primarily using the method of RAPD-PCR for selectively amplifying molecular markers of polymorphism from genomes of 12 local groups to clone representative molecular biomarker , and then synthesizing probe of DNA fragment by DNAⅠfollowed with Southern hybridization [RESULTS]was that the 10 local groups (represented especially as No1005) showed in common bands 700(bp) in the polymorphism map of genome. As opposed to the 10 groups, 2 local groups(represented especially as No1083) showed bands 700(bp) in faintness or did not show .[CONCLUSION]indicated that bands 700(bp)of fragment DNA ,homogeneous DNA, was preliminarily attributed to the genetic and could maybe be acted as specific molecular biomarker .
