Position: Home > Articles > Effect of treatment methods of hemolymph from shore crab Thalamita crenata on its paralytic shellfish poisoning toxicity neutralization
Journal of Fishery Sciences of China
2013,20
(6)
1219-1224
不同技术处理的钝齿短浆蟹淋巴液中和麻痹性贝毒毒性的效果
作 者：
陈晓;姜沛宏;林华娟
单 位：
水产品深加工广东普通高等学校重点实验室;广东省水产品加工与安全重点实验室;广东海洋大学食品科技学院
关键词：
钝齿短浆蟹;麻痹性贝毒(PSP);淋巴液;毒性中和效果
摘 要：
钝齿短浆蟹(Thalamita crenata)淋巴液可以中和麻痹性贝毒(PSP)的毒性,这个信息为PSP的降毒研究提供重要依据,但是淋巴液处理方法是否对其毒性中和效果有影响尚不清楚。本研究采用小鼠腹腔注射法,以淋巴液的PSP毒性中和效果为指标,探讨抗凝剂、提取方法和冻存条件等淋巴液处理方法对其PSP毒性中和效果的影响,为蟹淋巴液的制备提供科学依据。分析结果显示,不同抗凝剂对淋巴液的PSP毒性中和效果有不同程度的影响,其中EDTA抗凝剂对淋巴液的PSP毒性中和效果最好,相对于对照组(小鼠致死时间在5 min左右),PSP-EDTA-淋巴液组的小鼠均存活。低浓度EDTA(5 mmol/L)即可起到抗凝作用并保持淋巴液较好的PSP毒性中和效果,EDTA浓度过高反而对PSP毒性中和效果产生抑制作用。在低浓度EDTA条件下,针头抽取淋巴液的PSP毒性中和效果明显优于组织破碎法。EDTA浓度为5 mmol/L时,在针头抽取淋巴液组中,67%的小鼠致死时间超过30 min。相比之下,组织破碎提取的淋巴液组的小鼠致死时间均分布在8.1~24 min。另外,蔗糖对淋巴液的冻存起到较好的抗冷冻保护作用。以上结果表明淋巴液处理方法对其麻痹性贝毒毒性中和效果有较大影响。
译 名：
Effect of treatment methods of hemolymph from shore crab Thalamita crenata on its paralytic shellfish poisoning toxicity neutralization
作 者：
CHEN Xiao;JIANG Peihong;LIN Huajuan;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,College of Food Science and Technology,Guangdong Ocean University;
关键词：
Thalamita crenata;;paralytic shellfish poisoning(PSP) toxins;;hemolymph;;PSP toxicity neutralization
摘 要：
Hemolymph from shore crab,Thalamita crenata,is able to neutralize the toxicity of paralytic shellfish poisoning(PSP) toxins.This information offers a groundwork on investigating the detoxifcation of the PSP.Unfortunately,whether or not the treatment methods of the hemolymph will affect the PSP toxicity neutralization is still unclear.In this study,the effects of anticoagulants(EDTA,sodium oxalate,sodium citrate and heparin sodium),extracting methods(syringe needling extraction and tissue homogenizing extraction) and freezing storage condition(with or without sucrose) on PSP toxicity neutralization of the hemolymph were investigated through the mouse assay.The results showed that the mice intraperitoneal injection with the PSP mixed with EDTA-hemolymph were survival,while the mice's death times of hemolymph groups mixed with sodium oxalate,sodium citrate and heparin sodium were(27.86 ± 2.66) min,(28.89 ± 0.59) min and(14.08 ± 0.6) min,which to be 5.4,5.3 and 2.6 times to that of corresponding control,respectively.Low EDTA concentration was efficacious in anticoagulation of the hemolymph and preserving a good PSP neutralization,which seemed to be repressed with in presence of excessive EDTA.Hemolymph extracted by syringe needle showed higher PSP neutralization than that of extracted by tissue homogenization.While the EDTA concentration was at 5 mmol/L in 20 mmol/L phosphate buffer(pH 8.0),67% of the mice for the hemolymph group extracted by syringe needle died at more than 30 minutes.By comparison,the mice's death time for hemolymph group extracted by tissue homogenization ranged at 8.1 24.0 min.Moreover,sucrose could protect the hemolymph against from freeze denaturation.After freezing for 2 weeks,the PSP neutralization of the hemolymph with sucrose remained unchanged,while that of the hemolymph without sucrose decreased.These results indicate that the PSP neutralization of hemolymph was remarkably effected by anticoagulants,extraction methods and freezing storage condition.
导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
