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Position: Home > Articles > Studies on the Culture Conditions of Eucalyptus dunnii Subculture and Multiplication Chinese Agricultural Science Bulletin 2007,23 (12) 154-157

邓恩桉继代增殖培养条件研究初报

作  者：
易霭琴;童方平;宋庆安;李贵;丁婕;唐春
单  位：
湖南省湘潭县林业局;湖南省林木无性系育种重点实验室;中南林业科技大学;湖南省林业科学院
关键词：
邓恩桉;组织培养;继代;增殖系数;培养条件
摘  要：
为提高邓恩桉组培苗的增殖系数,降低组培苗生产成本,获得健壮的邓恩桉组培苗,通过不同基本培养基、谷氨酸、培养基不同pH值以及不同光照强度对邓恩桉继代培养增殖的影响试验,找到了邓恩桉的组织培养中继代增殖的适宜条件。结果表明,基本培养基、谷氨酸、pH值和光照强度对试管苗的继代增殖都会产生不同程度的影响。基本培养基以改良H为好,添加20mg/L的谷氨酸有利于试管苗的生长,pH值6.2最为适合,最合适的光照强度为2500lx。因此,适合邓恩桉继代增殖的培养条件为:改良H+BA0.2～0.4mg/L+NAA0.1～0.2mg/L+IBA0.2mg/L+LH500mg/L+谷氨酸20mg/L,pH6.2,光照强度2500lx。
译  名：
Studies on the Culture Conditions of Eucalyptus dunnii Subculture and Multiplication
作  者：
Yi Aiqing1,2, Tong Fangping1,2, Song Qing’an1,2, Li Gui 3, Ding Jie4,Tang Chun1,2 (1Hunan Forestry Academy, Changsha 410004; 2Trees Clones Breeding Technology Key Laboratory of Hunan Proninge, Changsha 410004; 3Central South University of Forestry and Technology, Changsha 410004; 4Forestry Bureau of Xiangtan County, Hunan Xiangtan 411228)
关键词：
Eucalyptus dununii, tissue culture, subculture , multiplication, culture conditions
摘  要：
In order to improve the multiplication coefficient of Eucalyptus dunnii, reduce the cost of culture and obtain the vigorous test-tube plantlet, culture conditions were optimized through testing the multiplication rate and height of test-tube plantlet under different conditions such as basic media, glutamic acid (Glu), pH, intensity of illumination. Through above research, this culture conditions influenced the subculture and multiplication of test-tube plantlet. Basic medium had effects on subculture, modified H was better than H, MS and modified MS. The culture media with Glu promoted the growth far more effectively than non- Glu ones. pH 6.2 and 2500 lx were the most suitable pH and intensity of illumination. So, the suitable culture conditions of Eucalyptus dunnii were modified H+BA 0.2 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L+LH 500 mg/L+ Glu20mg/L, pH 6.2, intensity of illumination 2500 lx.

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