作 者：
张韵;张金卫;易咏竹;李志勇;李江涛;鱼南洋;丁农;柳纪省
单 位：
湖州市农业科学研究院浙;中国农科院生物技术所;中国农业科学院兰州兽医研究所
关键词：
AsiaI口蹄疫病毒;P1-2A3C;家蚕品种
摘 要：
[目的]研究Asia I口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达,以期筛选出较适合表达该组合基因的家蚕品种。[方法]将已经获得高效表达的重组家蚕杆状病毒rBmNPV(P1-2A3C)基因,分别注射原种及杂交家蚕蚕蛹。利用ELISA方法对其进行抗原表达量检测,并对表达结果进行差异比较。[结果]不同家蚕品种对rBmNPV(P1-2A3C)的表达存在着明显差异;秋丰×TQ78和秋丰×丝胶茧杂交组合可考虑作为高效表达的家蚕生物反应器专用品种。[结论]为AsiaΙFMDV目的蛋白的高效表达专用品种的选育提供了依据。
译 名：
Expression of Recombinant Gene P1-2A3C of FMDV Asia I in Different Bombyx mori Varieties
作 者：
ZHANG Yun et al(Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gausu 730046)
关键词：
Asia I FMDV;P1-2A3C;Bombyx mori varieties
摘 要：
[Objective]The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expression.[Method]The recombinant B.mori baculovirus rBmNPV(P1-2A3C) are injected into pupae of original species and hybrids of B.mori respectively.Then,the expression of antigen is detected by ELISA method and compared.[Result]The expression level of P1-2A3C significantly differed in different B.mori varieties,in which,the hybrids of Qiufeng × TQ78 and Qiufeng × Sijiaojian could be considered as the effective bioreactors for high-level expression of foreign genes.[Conclusion]This study provided the basis for breeding special B.mori varieties which can highly express target protein of Asia Ι FMDV.
