当前位置: 首页 > 文章 > 不同培养条件对蓝浆果离体叶片不定芽再生的影响 北方园艺 2013 (22) 104-107
Position: Home > Articles > Effects of Different Culture Conditions on the Adventitious Bud Regeneration of Detached Leaves of Blueberries in vitro Northern Horticulture 2013 (22) 104-107

不同培养条件对蓝浆果离体叶片不定芽再生的影响

作  者:
张祝丽;姜燕琴;於虹
单  位:
江苏省中国科学院植物研究所
关键词:
蓝浆果;离体叶片;培养条件;不定芽再生
摘  要:
以南方高丛蓝浆果(Vaccinium corymbosumhybrids)品种"南月"(Southmoon)优选系A18离体叶片为试材,研究了不同细胞分裂素、蔗糖浓度、叶片放置方式、暗培养时间及叶片完整性对不定芽再生的影响。结果表明:TDZ和CPPU对不定芽的诱导效果好于ZT和2ip;在培养基中添加20g/L蔗糖较利于不定芽的再生;外植体近轴面接触培养基不定芽再生效果好;离体叶片不经过暗培养及其创伤口大均有利于不定芽再生。
译  名:
Effects of Different Culture Conditions on the Adventitious Bud Regeneration of Detached Leaves of Blueberries in vitro
作  者:
ZHANG Zhu-li;JIANG Yan-qin;YU Hong;Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences;
关键词:
blueberry;;detached leaves;;culture conditions;;adventitious bud regeneration
摘  要:
The detached leaves of superior strain A18 selected from southern highbush blueberry(Vaccinium corymbosum)‘Southmoon'were used as the explants,the effect of different cytokinin,sugar concentration,inoculation method,dark periods and artificial leaf wounds on adventitious bud regeneration was studied.The results showed that the effects of TDZ and CPPU were better than ZT and 2ip on adventitious bud regeneration;medium containing 20g/L sugar was suitable for adventitious bud regeneration;the adaxial side in contact with the medium had greater effect;no darkness treatments and artificial leaf wounds were also favorable to regeneration.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊