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Position: Home > Articles > On Application of Herbicide Resistant Gene bar and EPSPS in Transgenic Sugarcane Biotechnology Bulletin 2016,32 (3) 73-78

抗除草剂bar基因与EPSPS基因在转基因甘蔗中的应用研究

作  者:
王文治;杨本鹏;蔡文伟;熊国如;冯翠莲;王俊刚;武媛丽;沈林波;张树珍
单  位:
中国热带农业科学院甘蔗研究中心中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室
关键词:
转基因甘蔗;耐除草剂;bar基因;EPSPS基因;草铵膦;草甘膦
摘  要:
通过农杆菌介导法,分别将bar基因和EPSPS基因导入到甘蔗中,分别获得了抗草铵膦和草甘膦两种除草剂的转基因甘蔗。通过直接喷洒田间施用浓度的草铵膦和草甘膦除草剂,证明了转基因甘蔗T0、T1代、宿根1代和宿根2代外源基因遗传稳定,且均具有稳定的耐除草剂的功能。因此转耐除草剂基因的甘蔗,可以与转耐除草剂基因的玉米、大豆、油菜和棉花一样,有广阔的应用前景。
译  名:
On Application of Herbicide Resistant Gene bar and EPSPS in Transgenic Sugarcane
作  者:
WANG Wen-zhi;YANG Ben-peng;CAI Wen-wei;XIONG Guo-ru;FENG Cui-lian;WANG Jun-gang;WU Yuan-li;SHEN Lin-bo;ZHANG Shu-zhen;Sugarcane Research Center of CATAS,Institute of Tropical Bioscience and Biotechnology of CATAS,Key Biotechnology Laboratory for Tropical Crops of Ministry of Agriculture;
关键词:
transgenic sugarcane;;herbicide-resistant;;gene bar;;gene EPSPS;;glufosinate-ammonium;;glyphosphate
摘  要:
In this research gene bar and EPSPS were transferred into sugarcane cultivar via agrobacterium-mediated method,and the transgenic sugarcane lines resistant to glufosinate-ammonium and glyphosphate were obtained. Directly spraying two herbicides at application concentration for field onto the field planting the transgenic sugarcane lines demonstrated the genetic stability of exogenous gene in generation T0,T1,ratoon 1,and 2 of them,and the lines had the stable herbicide-resistant ability. Therefore,the transgenic sugarcane lines resistant to herbicide can be widely used in the field production as the other transgenic herbicide-resistant crops such as corn,soybean,rape and cotton.

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