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当前位置: 首页 > 文章 > 新扬州鸡IGF-Ⅰ基因多态性的PCR-SSCP检测及其与生长发育性状的相关性 福建农林大学学报(自然科学版) 2008,37 (6) 73-77
Position: Home > Articles > Detection of IGF-Ⅰ gene mutation with PCR-SSCP and the association between IGF-Ⅰ gene polymorphisms and growth character Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2008,37 (6) 73-77

新扬州鸡IGF-Ⅰ基因多态性的PCR-SSCP检测及其与生长发育性状的相关性

作  者：
王强;王志跃;杨海明;陈五湖;杨建刚
单  位：
陆良县活水镇动物食品站;扬州大学动物科学学院
关键词：
新扬州鸡;IGF-Ⅰ基因;PCR-SSCP
摘  要：
以新扬州鸡胰岛素样生长因子-Ⅰ(IGF-Ⅰ)基因作为控制其生长、发育性状的候选基因,通过对IGF-Ⅰ基因主要部分序列进行PCR-SSCP检测,发现了2个多态位点.测序结果表明,IGF-Ⅰ基因存在2个突变位点:插入(G)和(G→C).分析多态位点与0-12周龄鸡生长发育性状的关联性,结果表明:插入(G)突变基因型中,2周龄鸡体重BB型与AB型差异显著(P<0.05),但AA型与AB型和BB型差异均不显著,而(G→C)突变各基因型间没有显著差异;插入(G)突变位点对0-12周龄鸡的龙骨长、胫骨长均没有显著影响,而(G→C)突变位点仅在鸡6周龄时表现出与龙骨长、胫骨长间的差异显著性.
译  名：
Detection of IGF-Ⅰ gene mutation with PCR-SSCP and the association between IGF-Ⅰ gene polymorphisms and growth character
作  者：
WANG Qiang1,2,WANG Zhi-yue1,YANG Hai-ming1,CHEN Wu-hu1,YANG Jian-gang3(1.College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiansu 225009,China;2.Institute of Poultry Science,Chinese Academy of Agricultural Sciences,Yangzhou,Jiansu 225003,China;3.Animal Food Service Station,Huoshui,Luliang,Yunnan 655604,China)
关键词：
New Yangzhou chicken;IGF-Ⅰ gene;PCR-SSCP
摘  要：
Due to the importance of insulin-like growth factor-Ⅰ(IGF-Ⅰ) in regulating many physiological and metabolic processes in animal body,we applied PCR-SSCP to detect the mutation of IGF-Ⅰ gene in New Yangzhou chickens and to analyze DNA sequence.We gained two mutation loci of IGF-Ⅰ gene: insert (G),(G→C).The association between DNA mutation loci and chicken traits was analyzed with general line model.The results were as follows.In the insert(G) mutation genotypes,there was significant difference between body weights of 2 weeks aged BB and AB genotype(P<0.05),while there was no significant difference between AA and other genotypes.In the(G→C) mutation genotypes,there was no significant difference in body weight.Insert(G) mutation loci had no significant effect on fossil bone length and shank length of 0-12 weeks aged chickens,only(G→C) mutation loci had a significant effect on fossil bone length and shank length of 6 weeks aged chickens.

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