当前位置: 首页 > 文章 > 意大利蜜蜂蜂毒透明质酸酶基因的克隆与序列分析 浙江大学学报(农业与生命科学版) 2002,28 (3)
Position: Home > Articles > Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mellifera . Journal of Zhejiang University (Agriculture and Life Sciences) 2002,28 (3)

意大利蜜蜂蜂毒透明质酸酶基因的克隆与序列分析

作  者:
沈立荣;张传溪;程家安
单  位:
浙江大学应用昆虫学研究所
关键词:
意蜂;蜂毒;透明质酶;基因;序列分析
摘  要:
从意蜂毒腺抽提总 RNA,用 RT- PCR获得蜂毒透明质酸酶 (Am Hya)基因 ,其核苷酸序列全长为 114 9bp。将 Am Hya氨基酸序列与中蜂蜂毒透明质酸酶 (Ac Hya)和其它 5种透明质酸酶 (Hya)氨基酸序列比较 ,结果显示 ,Am Hya与 Ac Hya的同源性最高 (91% ) ,同时对 7种 Hya作了分子结构与分子进化分析
译  名:
Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mellifera .
作  者:
SHEN Li rong, ZHANG Chuan xi, CHENG Jia an(Inst. of Applied Entomolgy, Zhejiang University, Hangzhou 310029, China)
关键词:
Apis mellifera ; bee venom; hyaluronidase; gene; sequence analysis
摘  要:
The venomous hyaluronidase (AmHya) gene of Italian honey bee ( Apis mellifera ) collected in China was amplified by RT PCR from total RNA of venom glands of the worker bee. Sequence analysis showed the gene was composed of 1 149 nucleotides . The alignment of the amino acid sequence of AmHya gene and 6 Hya genes from other organisms showed that AmHya is most closely related to the AcHya of Chinese honeybee ( A.cerana cerana ) with 91% amino acid level identity. A phylogenetic tree of 7 Hyaluronidases was drawn by using GENETYX program, and molecular structures and functions of these ensymes were compared and analysed.

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