Position: Home > Articles > Effect of LED Different Light Qualities on Physiological and Biochemical Caracteristics of Cymbidium tracyanum var. huanghua×Cymbidium mastersii Tissue Culture Seedlings
Journal of Yunnan Agricultural University(Natural Science)
2012,27
(6)
863-869
LED不同光质对虎雪兰组培苗生理生化特性影响的研究
作 者：
石镇源;唐敏;杨红飞;王亚沉;张青华;张小平;李枝林
单 位：
云南农业大学花卉研究所;云南省电子工业研究所
关键词：
LED(发光二极管);光质;虎雪兰;组织培养
摘 要：
本试验在同等光强条件下研究LED不同光质对虎雪兰组培苗生理生化特性的影响,希望能够为植物生产类LED光源的参数设置和系统研发提供数据支持和理论依据。结果表明:(1)RBW(红∶蓝∶白=6∶1∶1)处理下虎雪兰组培苗整株干重最大,为0.098 2 g;对照组W1(白色荧光灯)根部干重最低,为0.082 3 g。(2)RBG(红∶蓝∶绿=4∶2∶1)处理下叶绿素a,叶绿素b和类胡萝卜素含量极显著高于其他处理(P<0.01),分别为0.438 0,0.159 0,0.091 0 g,利于虎雪兰中上述3种色素的合成。(3)B(单色蓝光)处理下叶片淀粉粒积累最少,为0.062 2 g,而其游离氨基酸和可溶性蛋白含量却是最高的,分别为140.997 1,5.001 4μg/g;而R(单色红光)处理下游离氨基酸和可溶性蛋白含量均最低,分别为99.455 7,2.726 9μg/g。综合比较其中RBG处理下虎雪兰组培苗长势最好,而R和B处理下长势相对较差,光质对虎雪兰幼苗生长代谢中的影响比较明显,LED光照系统可以替代荧光灯成为虎雪兰幼苗组织培养的理想光源。
译 名：
Effect of LED Different Light Qualities on Physiological and Biochemical Caracteristics of Cymbidium tracyanum var. huanghua×Cymbidium mastersii Tissue Culture Seedlings
作 者：
SHI Zhen-yan1,TANG Min1,YANG Hong-fei2,WANG Ya-chen1, ZHANG Qing-hua1,ZHANG Xiao-ping1,LI Zhi-lin1(1.Institute of Flowers,Yunnan Agricultural University,Kunming 650201,China; 2.Yunnan Institute of Electronics Industry,Kunming 650201,China)
关键词：
light-emitting diode(LED);light quality;Cymbidium tracyanum var huanghua×Cymbidium mastersii;tissue culture seedlings
摘 要：
In order to study growth metabolism influence of Cymbidium tracyanum var.huanghua×C.mastersii tissue culture seedlings under the same light intensity,to provide data support and theoretical basis of light-emitting diode(LED) light parameters settings and system research and development,the experiment was conducted.The results showed:(1) with treatment RBW(red∶ blue∶ white = 6∶ 1∶ 1),dry weight of the whole plant of seedlings cultivation was the biggest,with 0.098 2 g;Dry weight of the roots control group W1(fluorescent lamp or fluorescent) was minimum,accounting for 0.082 3 g.(2) RBG(red∶ blue∶ green = 4∶ 2∶ 1) with chlorophyll a,b and chlorophyll content of carotenoids significant was higher than other treatments,being 0.438 0,0.159 0,0.091 0 g respectively,and beneficial to the three pigments synthesis of seedlings.(3) B(monochrome blue-ray) accumulation of leaf starch grains was the least,with 0.062 2 g,and the free amino acids and soluble protein content was the highest,respectively 140.997 1,5.001 4 μg/g;And R(monochrome red light) with free amino acids and soluble protein content were the lowest,respectively 99.455 7,2.726 9 μg/g.Comprehensive comparison among them,RBG treatment was the best for seedlings growing,and R and B growing was poor relatively.C.tracyanum var.huanghua×C.mastersii tissue culture seedlings growth in the metabolism was influenced markedly by light quality,LED illumination system could replace fluorescent become a ideal light source in C.tracyanum var.huanghua×C.mastersii tissue culture seedlings tissue culture.
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