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Position: Home > Articles > Primary Studies on the Best Proposal for Testing Endogenous Hormones in Artemisia frigida Chinese Journal of Grassland 2012,34 (3) 117-120

冷蒿内源激素测定最佳方案初探

作  者:
李倩倩;宛涛;蔡萍;伊卫东;李姗姗
单  位:
内蒙古农业大学生态环境学院;锡林郭勒职业学院草原生态与畜牧兽医学院
关键词:
内源激素;高效液相色谱;冷蒿
摘  要:
探索了采用高效液相色谱法(HPLC)测定冷蒿内源激素——赤霉素(GA3)、脱落酸(ABA)、生长素(IAA)的方法,结果表明,以C18柱为分离柱,以甲醇∶1.8%冰乙酸∶水=30∶50∶20为流动相,在柱温35℃,波长254nm,流速1.0ml/min的检测条件下能快速、准确的测定冷蒿内源激素。
译  名:
Primary Studies on the Best Proposal for Testing Endogenous Hormones in Artemisia frigida
作  者:
LI Qian-qian1,WAN Tao1,CAI Ping2,YI Wei-dong1,LI Shan-shan1(1.College of Ecology and Environmental Science,Inner Mongolia Agricultural University, Hohhot 010019,China;2.College of Grassland Ecology and Animal Husbandry Veterinary Medicine, Xilinguole Vocational College,Xilinhaote 026000,China)
关键词:
Endogenous hormones;HPLC;Artemisia frigida
摘  要:
The endogenous hormones,ABA,IAA,GA3,were tested with High Performance Liquid Chromatography(HPLC) method.The results showed that endogenous hormones in Artemisia frigida can be rapid and accurately determined under the following conditions: C18 column as separation column,methanol∶1.8% ice acetic acid∶water = 30∶50∶20 as mobile phase,column temperature 35℃,wavelength 254nm,velocity 1.0ml/min.

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