中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 辽宁省西瓜炭疽病病原菌鉴定及生物学特性研究 植物保护 2014,40 (4) 38-44
Position: Home > Articles > Identification and biological characteristics of watermelon anthracnose caused by Colletotrichum orbiculare in Liaoning Province Plant Protection 2014,40 (4) 38-44

辽宁省西瓜炭疽病病原菌鉴定及生物学特性研究

作  者：
唐爽爽;刘志恒;余朝阁;郑川;安心;李健冰;赵廷昌
单  位：
中国农业科学院植物保护研究所;沈阳农业大学植物保护学院
关键词：
西瓜炭疽病;瓜类炭疽菌;鉴定;rDNA-ITS测序;生物学特性
摘  要：
针对西瓜炭疽病病原菌进行了鉴定及其生物学特性研究。通过病原菌形态学鉴定,柯赫氏法则证病,rDNAITS测序,鉴定该病致病菌为瓜类炭疽菌(Colletotrichum orbiculare)。生物学特性测定结果表明:病菌菌丝生长最适培养基为PSA;最适碳、氮源分别为蔗糖和NH4Cl;最适温度为25℃;最适pH为6;黑暗条件利于病菌生长。病菌产孢最适培养基为西瓜茎叶煎汁培养基;最适碳、氮源分别为蔗糖和(NH4)2SO4;最适温度为25℃;最适pH为6;黑暗条件利于孢子产生。菌丝致死温度为55℃,10min;分生孢子致死温度为52℃,10min。
译  名：
Identification and biological characteristics of watermelon anthracnose caused by Colletotrichum orbiculare in Liaoning Province
作  者：
Tang Shuangshuang;Liu Zhiheng;Yu Zhaoge;Zheng Chuan;An Xin;Li Jianbing;Zhao Tingchang;College of Plant Protection,Shenyang Agricultural University;Institute of Plant Protection,Chinese Academy of Agricultural Sciences;
关键词：
watermelon anthracnose;;Colletotrichum orbiculare;;identification;;rDNA-ITS;;biological characteristics
摘  要：
The watermelon anthracnose pathogens and their biological characteristics were studied.By morphological identification,card of Koch's postulation,rDNA-ITS sequencing,the watermelon anthracnose pathogen Colletotrichum orbiculare was identified.Biological characteristics measurement showed that PSA was the optimal medium for hyphal growth.The optimum carbon and nitrogen sources were sucrose and NH4Cl;the optimal temperature was 25℃;the optimal pH value was 6.The dark condition was conducive to bacterial growth.Fried juice of watermelon stems and leaves was the optimal culture medium for bacterial spores,and the optimum carbon,and nitrogen sources were sucrose and(NH4)2SO4;the optimal temperature was 25℃ and the optimal pH value was6;dark condition was conducive to spores.Mycelial lethal temperature was 55℃for 10 min,and conidial lethal temperature was 52℃for 10 min.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46