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Ultraviolet mutagenesis of Bacillus licheniformis LCB-8 and screening of high-yielding strain.

作  者:
Gao GuoLong;Min Zhang;Siqiang Li;Haiyan Wan
单  位:
College of Life Science,Guizhou Normal University,Guiyang 550001,China;College of Life Science,Guizhou Normal University,Guiyang 550001,China)
关键词:
strain;cellulase;amylase;dy;activities;protease
摘  要:
[Objective] To enhance amylase,protease and cellulase activities of Bacillus licheniformis LCB-8 by ultraviolet mutagenesis.[Method] Ultraviolet light was used to mutate this strain.The activities of amylase,protease and cellulase were determined to get high-yielding strains.[Result] The best time for ultraviolet mutation was 30 s,and the mortality rate reached 72.4%.After primary screening and rescreening,two better strains were obtained.After mutagenesis,the activities of amylase,protease and cellulase of DY-97 strain were enhanced by 150%,78% and 17% respectively.After mutagenesis,the activities of cellulase,amylase and protease of DY-34 strain were enhanced by 31%,46% and 64% respectively.[Conclusion] The DY-97 strain is suitable for fermentation of feed rich in protein and amylum,whereas the DY-34 strain is suitable for fermentation of feed rich in cellulose.

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