Wang Fang;Gao Zheng-liang;An Meng-nan;Zhou Ben-guo;Wu Yuan-hua

Anhui Acad Agr Sci, Tobacco Res Inst, Hefei 230031, Peoples R China;Shenyang Agr Univ, Coll Plant Protect, Shenyang 110866, Peoples R China

PVY Liaoning isolate;cloning;genome sequencing;phylogeny

Complete genome sequence of Potato virus Y Liaoning isolate (PVY-LN) causing tobacco vein necrosis symptoms were isolated from Liaoning Province in China. Genome sequences of PVY-LN was 9 714 nucleotides in length, excluding the 3'-terminal poly (A) tail. PVY-LN encodes a single long open reading frame (ORF) of polyprotein that is predicted to be cleaved into ten mature proteins by three viral proteases. No recombination can be predicted in PVY-LN sequences compared with that of the other PVY strains using Recombination Detection Programe v. 4.16 (RDP4). Complete genome sequence comparison and phylogenetic analysis indicated that PVY-LN is closely related to PVY necrosis strain (PVYN).