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东北农业大学学报(英文版)
2011,18
(2)
Position: Home > Articles > Cloning of Bile Salt Hydrolase Gene and Its Expression in Lactic Acid Bacteria
Journal of Northeast Agricultural University(English Edition)
2011,18
(2)
Cloning of Bile Salt Hydrolase Gene and Its Expression in Lactic Acid Bacteria
作 者：
Bin Li;Yujun Jian
单 位：
Key Laboratory of Dairy Science,Ministry of Education,College of Food,Northeast Agricultural University Harbin 150030,China;Key Laboratory of Dairy Science, Ministry of Education, College of Food, Northeast Agricultural University, Harbin 150030, China
关键词：
bsh;pnz8148;recombinant;lactic acid bacteria;expression vector;gen
摘 要：
According to the sequence of the bile salt hydrolase ( BSH ) gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase ( BSH ) gene was gotten from Bacillus bifidus ATCC 29521 by PCR. BSH gene was inserted into lactic acid bacteria expression vector pNZ8148 to construct the recombinant pNZ8148-BSH. The recombinant pNZ8148-BSH was transferred into lactic acid bacteria NZ9000 with electrotransformation method. And the recombinant which could express BSH protein was obtained. It was identified by SDS-PAGE electrophoresis and activity verification. The result could provide a rationale reference for expressing BSH in lactic acid bacteria.
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