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Position: Home > Articles > Culture and Immunohistochemistry Detection of Chicken's Pulmonary Arterial Smooth Muscle Cells Journal of China Agricultural University 2002,7 (3) 101-104

鸡肺动脉平滑肌细胞培养及免疫组化鉴定

作  者:
董世山;乔健;赵立红;孙茂红;张建军;田勇
单  位:
中国农业大学动物医学院
关键词:
鸡;肺动脉;平滑肌细胞;细胞培养;免疫组化;鉴定
摘  要:
应用组织贴块法、贴块配合差速贴壁法成功培养不同日龄鸡的肺动脉平滑肌细胞。一般需 4~ 7d细胞从组织块边缘长出 ,再经 5~ 1 0 d长成细胞单层。鸡日龄越小培养的肺动脉平滑肌细胞生长越旺盛。培养的肺动脉平滑肌细胞呈梭形、三角形 ,圆形核位于中央 ,胞浆丰富致密。用兔抗鸡α- actin多克隆抗体为一抗做免疫组化染色 ,平滑肌细胞占 95%以上
译  名:
Culture and Immunohistochemistry Detection of Chicken's Pulmonary Arterial Smooth Muscle Cells
作  者:
Dong Shishan Qiao Jian Zhao Lihong Sun Maohong Zhang Jianjun Tian Yong (College of Veterinary Medicine, China Agricultural University, Beijing100094, China)
关键词:
chicken; pulmonary; arterial smooth muscle cell; cell culture; immunohistochemistry; detection
摘  要:
The purpose of this experiment was to find some methods of culturing chicken's pulmonary arterial smooth muscle cells(PASMC). In this experiment, explants culture, explants culture combined with differential attachment were used. The primary culture of Chicken's PASMC was successfully done by using the two methods. The successive transfer culture was easily grasped. The α actin of cultured cells was detected by immunohistochemistry. The younger the chicken was, the easier the PASMC grows. The growing period of cells was about 10~15 d.

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