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当前位置: 首页 > 文章 > 两种杂交石斑鱼子一代及其亲本的线粒体COⅠ基因遗传变异分析 热带生物学报 2012,3 (1) 1-10
Position: Home > Articles > Analysis of Genetic Variability of mtDNA COⅠ Genes between two grouper hybrids and their parents Journal of Tropical Biology 2012,3 (1) 1-10

两种杂交石斑鱼子一代及其亲本的线粒体COⅠ基因遗传变异分析

作  者：
周翰林;杨森;高川;张磊;张海发;李水生;张勇;蒙子宁;刘晓春;林浩然
单  位：
广东省大亚湾水产试验中心;中山大学生命科学学院
关键词：
斜带石斑鱼;鞍带石斑鱼;棕点石斑鱼;杂交子代;COⅠ;遗传变异
摘  要：
对两种杂交石斑鱼子一代(青龙斑和虎龙斑)及其亲本(斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼)的线粒体DNA细胞色素氧化酶Ⅰ(COⅠ)基因序列进行了测序分析。在15个样本中,同源序列片段(1 551bp)中共检测到9个单倍型和249个核苷酸多态位点。序列差异分析和遗传距离比较结果显示,核苷酸序列同源性在88.1%～100%之间,无明显遗传分化。虎龙斑的2个COⅠ基因单倍型与母本棕点石斑鱼单倍型的同源性为99%和100%,而与父本鞍带石斑鱼的同源性均为88.1%。青龙斑的2个单倍型与母本斜带石斑鱼的3个单倍型的同源性在99.7%～100%之间,而与父本鞍带石斑鱼的同源性分别为89.3%和89.4%,结果表明两种杂交子一代在线粒体DNA COⅠ基因上严格遵循母性遗传规律。
译  名：
Analysis of Genetic Variability of mtDNA COⅠ Genes between two grouper hybrids and their parents
作  者：
ZHOU Han-lin1,YANG Sen1,GAO Chuan1,ZHANG Lei2,ZHANG Hai-fa2,LI Shui-sheng1 ZHANG Yong1,MENG Zi-ning1,LIU Xiao-chun1,LIN Hao-ran1,3(1.College of Life Sciences/Institute of Aquatic Economic Animals,Sun Yat-Sen(Zhongshan) University,Guangzhou 510275,China; 2.Guangdong Daya Bay Fisheries Development Center,Huizhou 516081,China; 3.College of Ocean,Hainan University,Haikou 570228,China.
关键词：
Epinephelus coioides;E.lanceolatus;Qinglong grouper;Hulong grouper;COⅠ;genetic variability
摘  要：
In order to identify the phylogenetic relationship among two grouper hybrids(Qinglong grouper and Hulong grouper) and their parents(Epinephelus coioides♀,Epinephelus coioides♀ and E.lanceolatus♂),their mitochondrial cytochrome oxidase subunit Ⅰ(COⅠ) gene fragments were amplified by PCR,and the 1551 base-pair nucleotide sequences of COⅠ were examined.The results show that all 15 sequences are grouped into 9 haplotypes and 249 nucleotide polymorphic loci in COⅠ gene fragments.Sequence divergences and genetic distance analysis showed all the groupers shared 88.1% to 100% similarities in nucleotide sequence,indicating there is no significant genetic difference among these groupers.The two COⅠ haplotypes of Hulong grouper showed 99% and 100% similarities with the haplotype of the female parent E.fuscoguttatus,respectively,but only 88.1% similarity with the male parent E.lanceolatus.The two COⅠ haplotypes in Qinglong grouper had similarities of 99.7% to 100% with the three haplotypes of the female parent E.coioides,and only of 89.3% and 89.4% with the male parent E.lanceolatus.The results indicated that the two grouper hybrids were inherited from their maternal parent in mitochondrial COⅠ genes.

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