当前位置: 首页 > 文章 > 3种山茶属植物花粉活力测定方法的比较 中南林业科技大学学报 2010,30 (3) 105-107
Position: Home > Articles > Comparison of detection methods for pollen viability of three kinds of Camellia plants Journal of Central South University of Forestry & Technology 2010,30 (3) 105-107

3种山茶属植物花粉活力测定方法的比较

作  者:
赵鸿杰;乔龙巴图;殷爱华;薛克娜;胡羡聪;卢永梅
单  位:
广东佛山市林业科学研究所;新疆乌苏市林业局
关键词:
植物生理学;张氏红山茶;越南抱茎茶;南山茶;花粉活力;染色法
摘  要:
采用I-IK法、过氧化物酶法和TTC法对张氏红山茶、越南抱茎、南山茶新鲜花粉的活力进行了测定。3种方法测定的结果表明:3种花粉活力都在92.00%以上,南山茶的花粉活力最高,在90.20%~100.00%,越南抱茎茶次之在80.80%~99.40%,张氏红山茶在84.80%~96.00%。3种方法所测花粉活力存在显著差异或极显著差异。I-IK染色法所测花粉活力高于过氧化物酶法和TTC法所测花粉活力,此法染色快,并且染色清晰,可用于山茶花粉活力的快速测定;过氧化物酶法次之,TTC法所测花粉活力最小,这2种方法,染色需时长,而且染色不清晰,不适于快速测定山茶花花粉活力。
译  名:
Comparison of detection methods for pollen viability of three kinds of Camellia plants
作  者:
ZHAO Hong-jie1,QIAOLONG Ba-tu2,YIN Ai-hua1,XUE Ke-na1,HU Xian-cong1,LU Yong-mei1(1.Foshan Institute of Forestry Science,Foshan 528222,Guangdong,China;2.Forestry Bureau of Wusu County,Wusu 833000,Xinjiang,China)
关键词:
plant physiology;Camellia brevipetiolata;Camellia amplexicaulis;Camellia semiserrata;pollen viability;coloration
摘  要:
The pollen viability of Camellia brevipetiolata,Camellia amplexicaulis,Camellia semiserrata were detected by methods of iodine-iodide kalium(I-KI),triphenyltetrazolium chloride(TTC) and peroxide enzyme(POD) coloration.Results show that pollen viability of Camellia semiserrata was the highest(97.80%~100.00%),that of Camellia amplexicaulis and Camellia brevipetiolata were 80.80%~99.40% and 84.80%~96.00% respectively.It can be concluded that I-KI methods was more favorable for detection than the other two because it got higher viability rate and can dye fast and clearly.

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