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当前位置: 首页 > 文章 > 苹果蛋白磷酸酶基因MdPP2C-Like的克隆及其对ABA的响应分析 园艺学报 2018 (7) 1225-1236
Position: Home > Articles > Molecular Cloning of Protein Phosphatase Gene MdPP2C-Like in Apple and the Response Analysis of ABA Acta Horticulturae Sinica 2018 (7) 1225-1236

苹果蛋白磷酸酶基因MdPP2C-Like的克隆及其对ABA的响应分析

作  者：
张春玲;周李杰;王桂栾;李媛媛;王小非;郝玉金
关键词：
苹果;MdPP2C-Like;蛋白磷酸酶;过表达;ABA
摘  要：
以‘嘎拉’苹果(Malus×domestica Borkh.)为试材,克隆了ABA信号转导过程中的响应基因蛋白磷酸酶基因MdPP2C-Like(基因序列号MDP0000126636)。基因结构分析显示,MdPP2C-Like定位于苹果8号染色体上,含有5个外显子与4个内含子;该基因全长1 035 bp,编码344个氨基酸,预测其蛋白质分子量为37.84 k D,等电点(p I)为5.72。蛋白质结构分析发现,其存在1个蛋白磷酸酶2C(PP2C)保守结构域,并与拟南芥中的A类PP2C保守结构域高度同源。构建的系统进化树表明MdPP2C-Like与At4G32950位于同一进化支,同源性较高。亚细胞定位结果表明,MdPP2C-Like主要定位于细胞核,少数分布在细胞质。定量分析显示,MdPP2C-Like在苹果不同组织中表达量不同,根中表达量较高。对获得的转基因拟南芥株系和苹果愈伤组织进行ABA响应分析,结果表明该基因的过量表达降低了植株和愈伤组织对ABA的敏感性。以上结果表明,克隆得到的MdPP2C-Like在响应ABA信号过程中起着重要作用。
译  名：
Molecular Cloning of Protein Phosphatase Gene MdPP2C-Like in Apple and the Response Analysis of ABA
作  者：
ZHANG Chunling;ZHOU Lijie;WANG Guiluan;LI Yuanyuan;WANG Xiaofei;HAO Yujin;College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,National Research Center for Apple Engineering and Technology;
单  位：
ZHANG Chunling%ZHOU Lijie%WANG Guiluan%LI Yuanyuan%WANG Xiaofei%HAO Yujin%College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,National Research Center for Apple Engineering and Technology
关键词：
apple;;MdPP2C-Like;;protein phosphatase;;overexpression;;ABA
摘  要：
Protein phosphatase gene MdPP2C-Like(MDP0000126636),an important factor that was involved in ABA signal pathway,was cloned from apple(Malus × domestica Borkh.). Gene structure analysis showed that MdPP2C-Like gene contained 5 exons and 4 introns,and it was located on the eighth chromosome of apple genome. After amplification,a 1 035 bp open reading frame(ORF)was obtained. MdPP2C-Like gene encoded a protein of 344 amino acids with a calculated mass of 37.84 kD and an isoelectric point(p I)of 5.72. MdPP2C-Like protein sequence comprised a conserved phosphatase 2 C(PP2C)domain,just as group A At PP2Cs. The phylogenetic tree of the construction indicated that MdPP2C-Like and At4 G32950 were located in the same evolutionary branch and had high homology. MdPP2C-Like gene mainly localized in the nucleus,with a few in cytoplasm. The expression level of MdPP2C-Like was different in all apple tissues,with the highest level in roots. Moreover,overexpression of MdPP2C-Like gene in apple callus and Arabidopsis plants reduced the sensitivity to ABA. Taken together,these findings showed that MdPP2C-Like played an important role in response to ABA signaling.

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