The Therapeutic Effect of Porcine Glucagon-like Peptide-2 in a Murine Model of Experimental Colitis

QI Ke-ke;WU Jie;XU Zi-wei;Institute of Animal Science,Zhejiang Academy of Agricultural Sciences;

pGLP-2;;DSS;;colitis;;mice

As an intestinal-specific trophic factor,porcine glucagon-like peptide-2(pGLP-2)could stimulate intestinal mucosal growth and reduce the severity of injury specificity.In order to provide a potential therapeutic agent for intestinal dysfunction and damage,the protective effects of pGLP-2for colonic injury were measured in dextran sulfate sodium(DSS)-induced colitis in mice.Eighteen BALB/c mice were randomly assigned to three treatments for 10days,mice of DSS group were fed 3%(w/v)DSS through their drinking water for 10days from first day of experiment,mice of DSS+pGLP-2group were extraperitoneally injected with pGLP-2daily from day 3to day 9and drink 3% DSS.The water group received water normally.Results were as follows:DSS-induced colitis clearly decreased body weight and relative expression of zonula occludens-1in the colon,which was significantly reduced by treatments with 3% DSS-pGLP-2.Administration of pGLP-2to DSS-mice preserved normal colonic mucosal architecture and significantly decreased myeloperoxidase activity,the relative expression of interleukin-6,interleukin-10and interferon-γwhich induced by DSS.These results showed that pGLP-2could reduce the severity of colonic in-jury in murine colitis.The potencies of this product highlight its potential as therapeutic agent for intestinal diseases in piglet.