Position: Home > Articles > IDENTIFICATION ON PORCINE HALOTHANE GENOTYPES BY POLYMEROSE CHAIN REACTION
Journal of Sichuan Agricultural University
1999,17
(1)
用PCR-RFLP技术鉴别猪的氟烷基因型
作 者：
郭雁君;罗安治;陈继华;肖艳玲;季永诚
单 位：
乐山市种畜场;四川农业大学动物科技学院
关键词：
猪,PCR－RFLP技术,氟烷基因
摘 要：
用ＰＣＲ－ＲＦＬＰ技术对乐山市种畜场长白猪选育基础群的氟烷基因型进行了检测，查清了Ｈａｌｎ基因在该群体中的分布，Ｈａｌｎ基因的频率为１８０５６％。同时对影响ＰＣＲ的几个主要因素进行了初步研究。
译 名：
IDENTIFICATION ON PORCINE HALOTHANE GENOTYPES BY POLYMEROSE CHAIN REACTION
作 者：
Guo Yanjun Luo Anzhi et al (College of Animal Science and Technology, Sichuan Agricultureal University/Yaan, Sichuan, China 625014)
关键词：
PORCINE STRESS SYNDROME HALOTHANE GENOTYPE POLYMERASECHAIN REACTION (PCR).
摘 要：
Porcine Stress Syndrome(PSS) is controlled by Halothanegene located on porcine No.6 chromosome. Identification of the porcineHalothane genotypes was established by PCR-RFLP. The Halothane genefragment containing the mutation point was amplified and the PCR product was incubated with restiction endonuclease HhaI, then genotypes were detected. And several factors that effected PCR are analysised.
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