摘 要:
以弥勒苣苔组培苗为试验材料,研究不同植物生长调节剂的不同浓度与基本培养基组合对弥勒苣苔组培苗生根的影响,并对诱导生根的组培苗在9种移栽基质中的移栽成活率和生长状况进行对比.结果表明,弥勒苣苔组培苗在1/2 MS+IBA 0.1 mg/L培养基中生根率达到89.6%,平均生根数量为4.3条,与其他处理差异极显著,为弥勒苣苔组培苗的最佳生根培养基;将生根苗移栽到基质 〔V(草炭)︰V(蛭石)︰V(珍珠岩)=1︰1︰1〕 中栽培,60 d后统计其移栽成活率达100%.所筛选出弥勒苣苔组培苗生根的最佳培养基和移栽的最佳基质,可为弥勒苣苔的种质保存及资源开发提供依据.
译 名:
Root Induction and Matrix Optimization of Paraisometrum mileense Tissue Culture Seedling
作 者:
ZHANG Lin-na;HE Jun;ZHANG Xiang;MENG Jing;College of Horticulture and Landscape,Yunnan Agricultural University;Kunming Institute of Botany,Chinese Academy of Sciences;
关键词:
Paraisometrum mileense;;tissue culture seedling;;rooting;;transplantation matrix
摘 要:
This study was to select the best shoot rooting medium and transplanting matrix of Paraisometrum mileense tissue culture seedling,in order to provide the basis for the conservation and development of this species.The effects of different concentration of various hormones with basic media on the roots induction were studied,and the survival ratio and growth of rooted plantlets in nine different transplanting substrates were also evaluated. The results showed that the most suitable rooting medium was 1/2 MS + IBA 0. 1 mg/L,on this medium the rooting rate and rooting number could be up to 89. 6% and 4. 3,and the survival rate of transplantation was about 100% for 60 days under the optimal transplantation matrix of V(peat)︰V(vermiculite)︰V(perlite) =1︰1︰1.