Position: Home > Articles > Determination of Human Serum Albumin(HSA) with Zn(Ⅱ)-Phen by Linear Sweep Polargraphy
Journal of Qingdao Agricultural University(Natural Science)
2009,26
(4)
352-355
锌(Ⅱ)-邻菲啰啉络合物线扫极谱法测定血清白蛋白的研究
作 者：
惠妮;王家胜;魏晓萍;孙伟
单 位：
南京农业大学工学院;青岛科技大学化学与分子工程学院;青岛农业大学化学与药学院
关键词：
锌(Ⅱ)-邻菲啰啉二元络合物;血清白蛋白;线扫极谱法;电化学探针
摘 要：
利用线扫极谱法研究了锌(Ⅱ)-邻菲啰啉(Phen)二元络合物与人血清白蛋白(HSA)的相互作用并建立了以Zn(Ⅱ)-Phen二元配合物为电化学探针测定HSA的分析测试方法。在最佳条件下,还原峰电流的降低值与人血清白蛋白的浓度2.0~20.0mg/L范围内呈线性关系,线性回归方程为ΔIp″(nA)=140.3C(mg/L)+1981.06(ni=16,γ=0.996),检测限为1.301mg/L(3σ)。应用该法测定了模拟样品和健康人血清样品中蛋白质的含量,结果令人满意。
译 名：
Determination of Human Serum Albumin(HSA) with Zn(Ⅱ)-Phen by Linear Sweep Polargraphy
作 者：
HUI Ni1,WANG Jia-sheng2,WEI Xiao-ping2,SUN Wei1(1.College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;2.College of Chemistry and Pharmacy,QAU)
关键词：
Zn-Phen complex;human serum albumin;linear sweep polarography;electrochemical probe
摘 要：
In this work,the interaction of Zn(Ⅱ)-Phen with HSA was investigated by linear sweep polarographic method,accordingly a new approach for HSA determination using Zn(Ⅱ)-Phen as an electrochemical probe was established.Under the optimal conditions,the reduction peak current of Zn(Ⅱ)-Phen decreases proportionally to the HSA concentration in the range of 2.0～20.0 mg/L,the linear regression equation was got as ΔIp"(nA)=140.3 C(mg/L)+1981.06(ni=16,γ=0.996) with the detection limit as 1.301 mg/L(3σ).The proposed method was further applied to determine the HSA content in the synthetic samples and in the healthy human serum samples with good results and good recovery.
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