中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 雷蒙德氏棉和亚洲棉SSR重复序列类型和丰度差异比较 棉花学报 2008,20 (6) 418-424
Position: Home > Articles > Comparison of SSR Types and Abundance Differences between G. raimondii and G. arboreum Cotton Science 2008,20 (6) 418-424

雷蒙德氏棉和亚洲棉SSR重复序列类型和丰度差异比较

作  者：
董薇;杜雄明;赖童飞
单  位：
中国农业科学院棉花研究所;农业部棉花遗传改良重点开放实验室
关键词：
雷蒙德氏棉;亚洲棉;EST拼接;SSR丰度
摘  要：
利用生物信息学相关方法对NCBI数据库中39277条亚洲棉(Gossypium arboretum L.)及63588条雷蒙德氏棉(Gossypium raimondiiL.)的EST序列共计64.08Mb(约相当于基因组的6.02%)分别进行拼接,比较分析了两棉种简单重复序列的重复类型及丰度差异。结果表明:拼接后的一致性序列中,亚洲棉比雷蒙德氏棉存在较高比例的单条序列簇;亚洲棉一至六碱基6种重复类型的SSR间隔距离分别比雷蒙德氏棉的大;亚洲棉中二到六碱基等5种重复类型的比例高于雷蒙德氏棉,但单碱基重复类型比例低于雷蒙德氏棉;两棉种的单碱基、四碱基和六碱基重复类型的优势重复基序和丰度也不同。因此,认为利用四、五、六碱基三种重复类型设计的SSR引物可以有效地鉴定亚洲棉和雷蒙德氏棉的基因组差异。对四倍体棉种的A、D亚组供体棉种中SSR的差异分析,为SSR标记开发、棉种起源和进化的深入研究提供更多有用的信息。
译  名：
Comparison of SSR Types and Abundance Differences between G. raimondii and G. arboreum
作  者：
DONG Wei,DU Xiong-ming,LAI Tong-fei(Cotton Research Institute,CAAS ;Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China)
关键词：
Gossypium raimondii;Gossypium arboreum;EST assembly ;SSR abundance
摘  要：
The 39277 G.arboreum and 63588 G.raimondii ESTs from NCBI databases which were in a total sizes of 64.08 Mb(approximately 6.02% of total cotton genome)were respectively assembled by bioinformatical methods,and the differences of Simple Sequence Repeat(SSR)types and abundance between these two species were analyzed.The main results were shown as follow:The singleton ratios of G.arboretum were higher than those of G.raimondii in the assembled tentative consensus sequences;The interval distances of six SSR types which were from mononucleotide to hexa-nucleotide repeats in G.arboreum were farther than those of the six SSR types in G.raimondii,respectively;The percentages of the five repeat types which were from di-to hexa-nucleotides were higher in G.arboreum than that of the five repeat types in G.raimondii.However,the percentages of mononucleotide repeat type were lower in G.arboreum.Moreover,the advanced SSR motifs and abundance of mono-,tetra-,and hexa-nucleotides repeat types were also different between two species.Therefore,it is suggested that the primers designed according to the tetra-or more nucleotides repeat types can effectively identify the genome differences between G.arboreum and G.raimondii.The differential analysis of SSR types and abundance in the donor species for A and D sub-genome of the tetraploid species provides more useful information for SSR marker design and profound research of the origin and evolution of Gossypium species.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46