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当前位置: 首页 > 文章 > 黄瓜发芽期耐热性评价及全基因组关联分析 植物遗传资源学报 2019 (2) 335-346
Position: Home > Articles > Evaluation and Genome-wide Association Study(GWAS)of Thermotolerance for Cucumber at the Germination Stage Journal of Plant Genetic Resources 2019 (2) 335-346

黄瓜发芽期耐热性评价及全基因组关联分析

作  者：
张松;苗晗;宋子超;薄凯亮;王烨;顾兴芳;张圣平
单  位：
中国农业科学院蔬菜花卉研究所
关键词：
黄瓜;核心种质;发芽期;耐热性;GWAS
摘  要：
高温会降低作物种子活力,减慢或完全抑制发芽,是制约黄瓜生产的重要非生物胁迫之一。本研究以96份黄瓜核心种质为试材,在42℃下进行发芽试验,利用相对发芽率、相对发芽势、相对发芽指数和相对胚根长度计算出的综合评价值,对核心种质发芽期耐热性进行鉴定评价;并利用重测序数据,对核心种质发芽期的耐热性进行全基因组关联分析。通过鉴定,筛选出了2份耐热性极强的材料CG109和CG110,综合评价值均大于0.9。通过全基因组关联分析,检测到5个与相对发芽率关联的位点,分别是GGP4.1、GGP4.2、GGP5.1、GGP5.2和GGR6.1;2个与相对发芽势关联的位点,分别是GGV4.1和GGV5.1;2个与相对发芽指数关联的位点,分别是GGI4.1和GGI5.1;2个与相对胚根长度关联的位点,分别是GRL6.1和GRL7.1。在以上位点中,GGI4.1、GGV4.1和GGP4.1均位于Chr.4的同一区间;GGI5.1、GGV5.1和GGP5.2均位于Chr.5的同一区间。这2个区段的关联位点利用相对发芽率、相对发芽指数和相对发芽势3个指标均可检测到,由此推测该区域存在与发芽期耐热性相关的基因。
译  名：
Evaluation and Genome-wide Association Study(GWAS)of Thermotolerance for Cucumber at the Germination Stage
作  者：
ZHANG Song;MIAO Han;SONG Zi-chao;BO Kai-liang;WANG Ye;GU Xing-fang;ZHANG Sheng-ping;The Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;
单  位：
ZHANG Song%MIAO Han%SONG Zi-chao%BO Kai-liang%WANG Ye%GU Xing-fang%ZHANG Sheng-ping%The Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
关键词：
cucumber;;core germplasm;;germination stage;;thermotolerance;;genome-wide association study
摘  要：
High temperature,which can reduce crop seed vigor,slow down or completely inhibit germination,is one of the important abiotic stresses that restrict cucumber production.A set of core germplasm including96 cucumber accessions were used to identify thermotolerance at high temperature 42 ℃ during the seed germination stage.The thermotolerance was evaluated using a comprehensive index,which include the relative germination percentage,relative germination vigor,relative germination index and relative radicle length.The re-sequencing data of the core germplasm were used to perform the Genome-wide association study(GWAS).We identified two accessions,CG109 and CG110,which have a pretty strong thermotolerance,with the comprehensive index of over 0.9.We detected 5 loci for relative germination percentage:GGP4.1,GGP4.2,GGP5.1,GGP5.2 and GGP6.1;2 for relative germination vigor:GGV4.1 and GGV5.1;2 for relative germination index:GGI4.1 and GGI5.1;2 for relative radicle length:GRL6.1 and GRL7.1.Among them,GGI4.1,GGV4.1 and GGP4.1 are located to the same physical region of Chr.4,and GGI5.1,GGV5.1 and GGP5.2 are located to the same region of Chr.5.The loci located in these two regions can be detected simultaneously by relative germination percentage,relative germination vigor and relative germination index three different indicators,which suggested that the two regions could include genes related to thermotolerance.

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