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普通荞麦愈伤组织诱导及其分化的正交设计试验研究

作  者:
王爱国;张以忠;任翠娟;陈庆富
单  位:
贵州师范大学生物技术与工程学院植物遗传育种研究所
关键词:
愈伤组织;普通荞麦;正交设计;组织培养
摘  要:
用正交设计法研究不同激素处理及不同外植体对普通荞麦愈伤组织诱导及分化过程中的影响。结果表明,在本实验设计中,2,4-D是诱导普通荞麦愈伤组织的主要因素;诱导愈伤组织的最佳培养基为MS+2.0 mg/L 2,4-D+1.5 mg/L6-BA;愈伤组织分化的主要因素是6-BA;愈伤组织分化的最佳培养基为MS+2.5 mg/L 6-BA+1.0 mg/L KT。
译  名:
Studies on Callus Induction and Differentiation in Fagopyrum Esculentum Moench by Orthogonal Design
作  者:
Wang Aiguo,Zhang Yizhong,Ren Cuijuan,Chen Qingfu(Institute of Plant Genetics and Breeding,College of Biological Technology and Engineering,Guizhou Normal University,Guiyang 550001)
关键词:
Callus Fagopyrum esculentum Moench Orthogonal design Tissue culture
摘  要:
Effects of various plant growth substances and different explants on callus induction and callus differentiation in Fagopyrum esculentum Moench were studied by orthogonal design statistic analysis.The results indicated that the main factor which influenced callus induction is 2,4-D,the optimal medium for callus induction is MS+2.0mg/L 2,4-D+1.5mg/L 6-BA;The results also showed that the main factor which influenced callus differentiation is 6-BA and the optimal medium for callus differentiation is MS+2.5mg/L 6-BA+1.0mg/L KT.

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