Position: Home > Articles > Determination of Chlorogenic Acid and Flavonoids in Stem and Leaf of Urtica dioica by HPLC
Hubei Agricultural Sciences
2012,51
(10)
2101-2103
HPLC法测定异株荨麻茎叶中绿原酸和黄酮类化合物
作 者：
何斌
单 位：
毕节学院环境与生命科学系
关键词：
异株荨麻(Urtica dioica L.);绿原酸;芦丁;槲皮素;高效液相色谱
摘 要：
建立了一种可同时测定异株荨麻(Urtica dioica L.)茎叶中绿原酸和黄酮类化合物的高效液相色谱法,色谱柱为钻石C18柱,流动相为甲醇和0.4%磷酸混合液(体积比为55/45),检测波长355 nm,柱温25℃,流速1.0 mL/min。结果表明,本法简便、快速、有效、准确、重现性好,可用于异株荨麻茎叶中绿原酸和黄酮类成分的定量分析。
译 名：
Determination of Chlorogenic Acid and Flavonoids in Stem and Leaf of Urtica dioica by HPLC
作 者：
HE Bin(Department of Environment and Life Science,Bijie University,Bijie 551700,Guizhou,China)
关键词：
Urtica dioica L.;chlorogenic acid;rutin;quercitrin;HPLC
摘 要：
HPLC method was established for determination of chlorogenic acid and flavonoids in the sample of Urtica dioica L.The sample was separated by Diamonsil C18 at 25 ℃ with the mobile phase of methanol and 0.4% phosphate acid(V/V=55/45),and detected at 355 nm.The flow rate was 1.0 mL/min.In a conclusion,the method is simple,effective,feasible and reproducible.It can be used to determine chlorogenic acid and flavonoids in U.dioica.
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