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当前位置: 首页 > 文章 > Bt棉不同种植年限土壤中Bt基因及其蛋白的残留测定 棉花学报 2008,20 (1) 81-83
Position: Home > Articles > Detection for Persistence of Bt Gene and Bt Insecticidal Proteins in Soil after Multiple Years of Bt Cotton Planting Cotton Science 2008,20 (1) 81-83

Bt棉不同种植年限土壤中Bt基因及其蛋白的残留测定

作  者：
沈平;张永军;陈洋;吴孔明;彭于发;郭予元
单  位：
中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
关键词：
Bt棉花;土壤;Bt杀虫蛋白;残留
摘  要：
从16个多年种植Bt棉花的田块抽取土壤样本。采用PCR和ELISA方法测定了土壤试样中Bt基因序列片段和杀虫蛋白的残留和累积效应。结果表明,以土壤总DNA为模板不能扩增出棉花内标准基因和cry1A基因预期大小片段。连续种植Bt棉花可以在土壤中低剂量残留Bt杀虫蛋白,但不会导致Bt毒蛋白在土壤中累积。
译  名：
Detection for Persistence of Bt Gene and Bt Insecticidal Proteins in Soil after Multiple Years of Bt Cotton Planting
作  者：
SHEN Ping1,2,ZHANG Yong-jun1,CHEN Yang1,WU Kong-ming1,PENG Yu-fa1,GUO Yu-yuan1(1.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China;2.Technology and Development Center of MOA,Beijing 100026,China)
关键词：
transgenic Bt cotton;soil;Bt insecticidal protein;persistence
摘  要：
Soil samples were collected from within sixteen fields where insect-resistant transgenic cotton encoding the cry1A gene had been grown and subsequently incorporated into soil by post-harvest tillage for multiple years.The cry1A gene sequence fragments and Bt insecticidal protein in these samples(collected after the last season tillage)were explored using PCR amplification and enzyme-linked immunosorbent assay(ELISA)method,respectively.Soil total DNA could be gotten in high quality,but we could not get any PCR amplification result of cotton internal standard gene or cry1A gene.The results from ELISA indicated that repeated agricultural using of transgenic Bt cotton expressing Cry1A insecticidal protein could persist extremely low levels of Bt protein in soil.However,the persistence of Bt protein in soil did not result in accumulation by post-harvest tillage in multiple seasons.

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