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当前位置: 首页 > 文章 > 基于CHD基因和EE0.6基因遗传多态鉴定白枕鹤、丹顶鹤性别研究报告 野生动物学报 2018 (1) 151-153
Position: Home > Articles > Gender Identification of White-Naped Crane and Red Crowned Crane Based on Genetic Polymorphism of EE0.6 Gene and CHD Gene Chinese Journal of Wildlife 2018 (1) 151-153

基于CHD基因和EE0.6基因遗传多态鉴定白枕鹤、丹顶鹤性别研究报告

作  者：
孙志明;徐琪;徐禄;倪谷音;王金美
单  位：
扬州大学动物科学与技术学院;无锡市动物园管理处
关键词：
白枕鹤;丹顶鹤;CHD基因;EE0.6序列;性别鉴定
摘  要：
白枕鹤(Grus vipio)和丹顶鹤(Grus japonensis)是中国重点保护动物,为单态性鸟,很难通过外观和形态来进行性别鉴定,给人工繁殖造成很大困难。研究已发现CHD基因和EE0.6序列片段大小在非平胸鸟类W、Z染色体上均存在差异。基于此,本研究以无锡市动物园饲养的白枕鹤和丹顶鹤为研究对象,经PCR实验,对白枕鹤和丹顶鹤CHD基因和EE0.6序列的相关片段进行了特异性扩增。结果显示,这2种方法 PCR扩增结果一致,并根据这一结果进行白枕鹤、丹顶鹤配对,均成功孵化出后代,表明该PCR鉴定结果可靠,研究结果为提高鹤类人为配对指数和繁殖率奠定了基础。
译  名：
Gender Identification of White-Naped Crane and Red Crowned Crane Based on Genetic Polymorphism of EE0.6 Gene and CHD Gene
作  者：
Sun Zhiming;Xu Qi;Xu Lu;Ni Guyin;Wang Jinmei;Jiangsu Wuxi Zoo;Jiangsu Yangzhou University College of Animal Science and Technology;
关键词：
White-naped crane;;Red-crowned crane;;CHD gene;;EE0.6 gene;;Gender identification
摘  要：
White-naped crane( Grus vipio) and red crowned crane( Grus japonensis) are national protected species in China. They are sexually monomorphic species and it is difficult to identify gender based on their external morphology. This causes difficulties in artificial propagation. Studies have found that there are sequence fragment size difference in the CHD and EE0. 6 genes on the W and Z chromosome of non-ratite birds. In this study, the fragments of CHD and EE0. 6 genes of white-naped and red-crowned cranes at Wuxi City Zoo were amplified by PCR. The results of two methods were consistent and were used to pair cranes of both species. The pairings resulted in successful production of offspring. The results of PCR gender determination were reliable. This study laid a foundation for increasing the artificial pairing index and reproduction rate of captive cranes.

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