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Position: Home > Articles > O3浓度升高和UV-B辐射增强对大豆叶片叶绿素含量和活性氧代谢的影响 Chinese Journal of Applied Ecology 2013,24 (5) 1277-1283

O3浓度升高和UV-B辐射增强对大豆叶片叶绿素含量和活性氧代谢的影响

作  者:
赵天宏;刘轶鸥;王岩;刘波;赵艺欣;曹莹
单  位:
沈阳农业大学农学院
关键词:
O3浓度升高;UV-B辐射增强;大豆;活性氧;叶绿素
摘  要:
以大豆栽培品种铁丰29为试验材料,利用开顶式气室研究O3浓度升高和UV-B辐射增强复合胁迫对大豆叶片叶绿素(Chl)含量、膜脂过氧化程度、活性氧产生速率、抗氧化酶活性和籽粒产量的影响.结果表明:在大豆整个生育期内,与对照相比,O3和UV-B单一胁迫及其复合胁迫下的大豆叶片Chl(a+b)、Chl a和Chl b含量均呈下降趋势;相对电导率、丙二醛含量增大,活性氧产生速率和H2O2含量增加,超氧化物歧化酶、过氧化物酶和过氧化氢酶活性下降,产量降低.O2和UV-B复合胁迫加剧了大豆叶片膜脂过氧化程度,促进大豆体内活性氧自由基的产生,使大豆抗氧化能力减弱,叶绿素含量降低,对大豆表现为协同效应.O3胁迫对大豆叶片的影响与复合胁迫更相近,其原因可能是在复合胁迫中臭氧起主要作用.
单  位:
College of Agronomy,Shenyang Agricultural University,Shenyang 110866,China
关键词:
elevated O_3 concentration%elevated UV-B radiation%soybean%active oxygen%chlorophyll
摘  要:
Taking the soybean(Glycine max) cultivar Tiefeng 29 as test material,and by using open-top chamber,this paper studied the effects of elevated O3 concentration and UV-B radiation on the leaf chlorophyll content,lipid peroxidation,reactive oxygen species(ROS) production rate, anti-oxidation enzymes activities,and the grain yield.During the growth period of soybean,as compared with the control,the leaf Chl a,Chl b and Chl(a+b) contents under the stresses of O3 and UV-B had a decreasing trend,the relative electrical conductivity,malondialdehyde content,superoxide anion(O2-) production rate,and hydrogen peroxide(H2O2) content increased,and the activities of superoxide dismutase,peroxidase and catalase as well as the grain yield decreased.O3 + UV stress aggravated the leaf membrane lipid peroxidation,promoted the ROS production,and decreased the plant antioxidant capacity and leaf chlorophyll content.The negative effects of O3 stress on soybean leaves were more close to the impacts of O3+UV stress,suggesting that O3 might play an important role in the combined stress.

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