单 位：
中国农业大学植物保护学院;青岛农业大学菌根生物技术研究所
关键词：
AMF;PGPR;生姜青枯病;菌根侵染率;病情指数;防御性酶
摘 要：
丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)与植物根围促生细菌(Plant growth-promoting rhizobacteria,PGPR)占据相近的生态位,对植物病原物及其所致病害的发生与发展具有重要影响。本试验旨在于温室盆栽条件下探索AMF摩西管柄囊霉(Funneliformis mosseae,Fm)、根内球囊霉(Glomus intraradices,Gi)和地表球囊霉(Glomus versiforme,Gv)与PGPR假单胞菌(Pseudomonus sp.)S1-10菌株和S3-11菌株的相互作用及其对生姜(Zingiber officinale)青枯病(Ralstonia solanacearum,RS1)的影响。试验结果表明Gv显著增加假单胞菌S3-11菌株在生姜根围的定殖数量(P<0.05),除生姜幼苗期外Fm则能促进S3-11菌株和S1-10菌株在根围内的定殖。PGPR S1-10和S3-11显著促进发棵期和块茎膨大期生姜AMF的侵染;发棵期S1-10显著提高了Gi的侵染率,但显著降低了Gv的侵染率(P<0.05);块茎膨大期S3-11对Gv侵染(64%)的促进作用最大。AMF或/和PGPR(除S1-10外)接种处理均不同程度地促进了生姜的生长,其中Gv+S3-11组合处理的生姜生长量最大,其次为Fm+S3-11组合。无论是单接种还是双接种,供试PGPR和AMF均显著提高叶片防御性酶超氧物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸(PAL)和过氧化氢酶(CAT)活性,降低丙二醛(MDA)含量和生姜青枯病的病情指数,其中,以Gv+S3-11组合处理的病情指数最低(25.5),且防效最高(71%)。研究结果表明AMF地表球囊霉与PGPR假单胞菌S3-11菌株组合能够相互促进、协同抑制生姜青枯病菌、诱导生姜抗病性、促进生姜生和增加产量,是适宜生姜生长的优良AMF+PGPR组合。
译 名：
Effects of Arbuscular Mycorrhizal Fungi and Plant Growth-Promoting Rhizobacteria on Bacterial Wilt of Ginger
作 者：
LIU Gui-meng;TAN Shu-peng;SUN Wen-xian;LIU Run-jin;Institute of Mycorrhizal Biotechnology,Qingdao Agricultural University;College of Agronomy and Biotechnology,China Agricultural University;
关键词：
arbuscular mycorrhizal fungi;;plant growth-promoting rhizobacteria;;ginger bacterial wilt;;colonization rate;;disease indexes;;defensive enzymes
摘 要：
Arbuscular mycorrhizal fungi( AMF) and plant growth-promoting rhizobacteria( PGPR)occupy the similar niche,and affect the occurrence and development of plant disease. The purpose of the present experiment was to estimate the effects of AMF Funneliformis mosseae( Fm),Glomus intraradices( Gi),and Glomus versiforme( Gv) and PGPR Pseudomonus sp. S1-10 or S3-11 on bacterial wilt( Ralstonia solanacearum,RS1) of ginger( Zingiber officinale) grown in pots under greenhouse conditions. Gv significantly increased numbers of S3-11 colonization in the rhizosphere soil,Gm also enhanced the colonization of both S1-10 and S3-11 in tillering and maturing periods. PGPR S1-10 and S3-11 significantly enhanced colonization of AMF on ginger plants in the tillering period and tuber maturing period. S1-10 significantly increased the colonization percentage of Gi on ginger in the tillering period,but significantly decreased the colonization percentage of Gv( P<0.05). And S3-11 had the greatest effect in increasing Gv colonization( 64%) in tuber maturing period. Except S1-10,all the tested AMF species or/and PGPR isolate inoculation treatments improved the growth of ginger plants in various degree,Gv+S3-11 combination showed the greatest effects,followed by Fm+S3-11 combination. Inoculations with single AMF species or PGPR isolate or with the combination of AMF and PGPR significantly increased the activities of defensive enzymes( SOD,POD,CAT and PAL),and decreased malondialdehyde contents in leaves and the wilt disease indexes of ginger plants. Gv+S3-11 combination showed the lowest disease index( 25.5) and highest control effects( 71%). It suggests that the combination of Glomus versiforme and PGPR Pseudomonus S3-11 synergically retard the wilt bacteria,induce plant disease resistance and improve ginger growth and yield. It should be one of the superiority of AMF+PGPR combination suitable for ginger production,and further research is needed to develop biofertilizer and biocide resources.
