Position: Home > Articles > Biological Characters of A Porcine-origin Imipenem-resistant Bacterium Strain
Journal of Yunnan Agricultural University(Natural Science)
2013,28
(2)
201-204
一株猪源沃氏葡萄球菌的分离鉴定及耐药特性分析
作 者：
匡雪;杨国淋;陈培富
单 位：
云南农业大学动物科学技术学院
关键词：
超广谱耐药性;沃氏葡萄球菌;16S rRNA;亚胺培南;猪源细菌
摘 要：
采用含16μg/mL亚胺培南选择剂的MH琼脂培养基,从外观健康猪新鲜粪便中分离培养获得1株耐亚胺培南细菌。该菌镜检为粗大的球杆状革兰氏阳性菌,生化发酵赖氨酸、七叶苷和木糖醇均为阳性,对10种常规抗生素均呈高度耐药,具有"超级细菌"的特点。经聚合酶链式反应(PCR)扩增、克隆及测序分析该菌株16S rRNA,将其初步鉴定为沃氏葡萄球菌(Staphylococcus warneri)。鉴于猪与人类健康关系密切,该结果提示应加强对猪源细菌耐药性监测及基础研究。
译 名：
Biological Characters of A Porcine-origin Imipenem-resistant Bacterium Strain
作 者：
KUANG Xue,YANG Guo-lin,CHEN Pei-fu(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)
关键词：
super broad-spectrum resistance;Staphylococcus warneri;16S rRNA;imipenem;porcine-origin bacterium
摘 要：
An imipenem-resistant bacterium strain was isolated from the fresh feces of apparently healthy pigs using Mueller-Hinton(MH) agar containing 16 μg/mL imipenem as a selective reagent.The obtained bacterium was a rough Gram-positive coccus-baccillus under the microscope and fermented lysine,aescin glucoside and xylitol,and showed strong resistance to 10 conventional antibiotics and was thus characteristic of super bacterium.Through polymerase chain reaction(PCR) amplification,cloning and sequencing of this isolate's 16S rRNA,it was preliminarily identified as Staphylococcus warneri.Given that pigs are closely concerned with human health,the results suggest that the monitoring and basic research of porcine-origin bacterial resistance to antibiotics should be emphasized.
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