Effects of SB-431542 on the Proliferation of Sika Deer Antler Chondrocytes

Han Yushuai, Zhang Lu, Zhao Lihong, Guo Bin, Feng Haihua, Yue Zhanpeng ( College of Animal Science and Veterinary Medicine , Jilin University , Changchun 130062)

SB-431542; sika deer antlers; chondrocyte; proliferation

The regulatory mechanism of TGF-β on the rapid growth of antler was investigated by studying the effect of specific micro-molecule antagonist SB-431542 of transforming growth factor- β (TGF-β) on the cell proliferation of sika antler chondrocytes. The sika deer antler chondrocytes were isolated at the 30th day of the growth, then the second generation cells were incubated with 0, 1, 3, 5, 8, 10 μmol/L of SB-431542, after 48 hours of culture, the MTT assay was used to examine the proliferation of antler chondrocytes and the SPSS software was used to analyze the difference of the results. The result showed that the proliferation activity of cells treated with SB-431542 was lower than that of the control group (P<0.05), among them, the proliferation activity of cells treated with SB-431542 at the concentration of 8 μmol/L and 10 μmol/L was significantly lower than that of the control (P<0.01), with the increased concentration of SB-431542, the inhibition to cell proliferation rate was more significant. In conclusion, these results indicated that TGF-β might play an important role to maintain the rapid proliferation of sika antler chondrocytes.