作 者：
杨泽宏;杨星勇;袁小红;罗克明;罗小英;张永军;郭余龙;裴炎
摘 要：
用昆虫生理盐水浸提小皱蝽成虫 ,浸提液冻干后 ,酸提取法制备粗提液 ,固相萃取 (SPE)初分级分离 ,活性洗脱组分反相高效液相色谱 (RP -HPLC)纯化 ,进一步凝胶渗透色谱 (GPC)及反相高效液相色谱 (RP -HPLC)纯化 ,分离得到一抗菌肽 ,茚三酮反应黄色 ,命名为AMP - 1。在 190nm～ 2 2 5nm短波紫外区 ,AMP - 1有紫外吸收。
译 名：
PURIFICATION OF THE ANTIMICROBIAL PEPTIDE AMP-1 OF CYCLOPELTA PARVA DISTANT BY HPIC
作 者：
YANG Ze-hong,YANG Xing-yong *,YUAN Xiao-hong,LUO Ke-ming LUO Xiao-ying,ZHANG Yong-jun,GUO Yu-long,PEI Yan (Biotechnology Center,Southwest Agricultural University,Chongqing 400716,China)
关键词：
HPLC;purification; Cyclopelta parva Distant;antimicrobial peptide
摘 要：
An antimicrobial peptide,AMP-1,was isolated from the bug Cyclopelta parva Distant,an insect of Pentatomidae,Hemiptera,by the extraction and preparation of the crude extract with the acid extraction methods and fractionation of Sep-Pak C 18 Cartridge.Subsequently,40% Sep-Pak fraction was purified by the initial reverse phase HPLC.The active peak was further fractionated by gel-permeation chromatography HPLC and the final reverse phase HPLC.All the HPLC purifications utilized the Acetonitrile-Water-Trifluoroacetic acetic elution system as mobile phase,with detection at 225 nm.The pure substances were recovered and the anti-bacterial activity of each aliquot was routinely monitored by the plate growth inhibition assay on S.aureus and E.coli, AMP-1 is the antimicrobial polypeptide.
