当前位置: 首页 > 文章 > 黄山贡菊茎尖脱毒快繁技术研究 北方园艺 2014 (1) 106-108
Position: Home > Articles > Tissue Culture and Rapid Propagation of Chrysanthemum morifolium Northern Horticulture 2014 (1) 106-108

黄山贡菊茎尖脱毒快繁技术研究

作  者:
李云玲;孙虎;苗锦山;刘杰;孙明茂;孙海亮
单  位:
潍坊科技学院园艺科学与技术研究所;山东禾宜生物科技公司
关键词:
黄山贡菊;茎尖;组织培养;快繁
摘  要:
以黄山贡菊茎段为外植体,研究了不同消毒方法和不同培养基对黄山贡菊茎尖组织培养的影响。结果表明:0.1%升汞消毒8min,外植体污染率及褐化率较低;诱导茎尖分化的最适培养基为MS+6-BA 1.0mg/L+NAA 0.05mg/L,芽诱导率可高达100%,且丛生芽生长较快;最适宜生根的培养基为MS+NAA 0.5mg/L,植株生根粗壮,根系发达,利于练苗及移栽。
译  名:
Tissue Culture and Rapid Propagation of Chrysanthemum morifolium
作  者:
LI Yun-ling;SUN Hu;MIAO Jin-shan;LIU Jie;SUN Ming-mao;SUN Hai-liang;Institute of Horticultural Science and Technology,Weifang University of Science and Technology;Shandong Heyi Bio-technology Corporation;
关键词:
Chrysanthemum morifolium;;stem tip;;tissue culture;;rapid propagation
摘  要:
With the stem tips of Chrysanthemum morifoliumas the explants,the effects of different disinfection methods and culture mediums on stem tip tissue culture were studied.The results showed that the contamination rate and browning rate of the explants were the lowest by 0.1% mercuric chloride for 8min.The optimum culture medium for inducing adventitious shoots was MS+6-BA 1.0mg/L+NAA 0.05mg/L,the bud induction rate was up to 100%,and the multiple shoots grow rapidly;the optimum rooting culture medium was MS+NAA 0.5mg/L,it was easier for acclimatizing and transplanting with the sturdy root system.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊