Position: Home > Articles > CULTURE AND MATURATION IN VITRO OF BOVINE FOLLICULAR OOCYTES AFTER DEEP FREEZING AND THAWING
Jiangsu Journal of Agricultural Sciences
1985
(2)
27-32
牛卵泡卵母细胞超低温冷冻和解冻后的体外培养成熟
作 者：
范必勤;M.T.Ridha;W.R.Dukelow
单 位：
江苏省农业科学院畜牧兽医研究所
关键词：
牛卵泡卵母细胞;超低温冷冻;体外培养
摘 要：
本研究目的在于探索牛卵泡卵母细胞的耐冻性和解冻后体外成熟的可能性。利用从屠宰母牛卵巢采集的1407个卵母细胞在四种冷冻液中进行超低温冷冻保存。解冻后,卵母细胞的形态正常率和存活率分别为88.1％和86.5％。四种冷冻液中卵母细胞的存活率无显著差异(P>0.05)。然而,慢解冻的卵母细胞存活率显著高于快解冻者(P<0.01)。经冷冻保存和解冻后尚存活的牛卵泡卵母细胞作体外培养时,有一定比例(15.1％)出现第一极体达到成熟阶段。研究结果证明,牛卵巢卵母细胞可作超低温冷冻长期保存,解冻后可以体外培养成熟。
译 名：
CULTURE AND MATURATION IN VITRO OF BOVINE FOLLICULAR OOCYTES AFTER DEEP FREEZING AND THAWING
作 者：
Fan Biqin M.T.Ridha W.R. Dukelow
关键词：
Bovine follicular oocytes; Deep freezing; Culure in vitro
摘 要：
The present study was undertaken to examine the effects of freeze-thawing procedures on the survival of bovine follicular oocytes and their ability to mature in vitro. A total of 1407 oocytes collected from follicular aspiration were frozen in four media (TC-199( II)+20% FCS, Tyrode's solution+20%FCS, Dulbecco's PBS+20%FCS and BFFM+20%FCS) containing 1.5MDMSO at 0.28℃/min to-80℃and then plunged in liquid nitrogen and preserved for 15.7 days (1-41 days) before thawing. The samples were thawed slowly at 0.7℃/min from-120℃ to 0℃ or quickly at 93℃/min from -196℃ to 0℃. After thawing, the rates of normal and survival oocytes were 88.1% and 86.5% respectively. There was no significant difference in the maturation rate of oocytes between the 4 media (P > 0.05). But a highly significant difference was found in oocyte maturation rate between the two thawing methods (P< 0.01). The proportion of oocytes exhibiting primary polar body formation was 15.1% when the frozen-thawed oocytes were cultured in vitro. These results indicate that bovine follicular oocytes can be frozen for long term preservation in liquid nitrogen and mature in vitro after thawing.
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