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当前位置: 首页 > 文章 > 成年牦牛和雄性不育犏牛睾丸eIF4G3 mRNA水平的比较 黑龙江畜牧兽医 2016 (15) 208-211+298
Position: Home > Articles > Comparison of the mRNA expression levels of eIF4G3 gene in the testes of adult yaks and sterile cattle-yaks Heilongjiang Animal Science and Veterinary Medicine 2016 (15) 208-211+298

成年牦牛和雄性不育犏牛睾丸eIF4G3 mRNA水平的比较

作  者：
雷杰雯;黄林;杨乐;金素钰;郑玉才
单  位：
西南民族大学生命科学与技术学院
关键词：
牦牛;犏牛;e IF4G3基因;定量PCR;睾丸
摘  要：
为了比较牦牛和犏牛睾丸组织中真核起始因子e IF4G3基因的相对表达量,探讨e IF4G3基因表达与犏牛雄性不育的关系,试验从牦牛、犏牛睾丸组织中提取总RNA,采用实时荧光定量PCR技术检测牦牛和犏牛睾丸组织中e IF4G3基因mRNA水平。结果表明:牦牛睾丸中e IF4G3基因的表达量极显著高于犏牛(P<0.01)。说明了e IF4G3作为一个重要的翻译调控因子,可能与犏牛睾丸组织中蛋白质表达的改变有一定关联。
译  名：
Comparison of the mRNA expression levels of eIF4G3 gene in the testes of adult yaks and sterile cattle-yaks
作  者：
LEI Jiewen;HUANG Lin;YANG Le;JIN Suyu;ZHENG Yucai;College of Life Science and Technology,Southwest University for Nationalities;
关键词：
yak;;cattle-yak;;e IF4G3 gene;;quantitative PCR;;testis
摘  要：
To compare the relative expression levels of eukaryotic initiation factor(e IF) 4G3 gene in the testis tissues of yak and cattle-yak,and explore the relationship between e IF4G3 gene expression and cattle-yak male sterility,total RNA was extracted from the testis tissues of yak and cattle-yak.The mRNA levels of the e IF4G3 gene in the testis tissues of yak and cattle-yak were detected using real-time fluorescent quantitative PCR technology.The results showed that the mRNA expression level of e IF4G3 gene in the testis of yak was extremely significantly higher(P < 0.01) than that of cattle-yak.The result indicates that e IF4G3 as an important translation regulatory factor,may be definitely associated with the changes of protein expression in cattle-yak testis tissue.

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