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Position: Home > Articles > Development and Application of PCR Assay for Detection on Fowl Adenovirus 4 China Animal Health Inspection 2016,33 (5) 72-74

禽腺病毒4型PCR检测方法的建立与应用

作  者:
袁万哲;张姗;陈萍;张超;经美;崔元;孙继国;刘聚祥
单  位:
石家庄市兽用生物制品供应站;河北农业大学动物医学院
关键词:
禽腺病毒4型;PCR;检测
摘  要:
根据禽腺病毒4型(FAdV-4)Hexon基因核苷酸序列,设计用于扩增FAdV-4的PCR引物,建立了FAdV-4 PCR检测方法。特异性和敏感性实验结果表明,该方法可扩增出954 bp的特异性核酸片段,而对禽流感病毒、新城疫病毒、传染性支气管炎病毒与禽腺病毒11型的检测结果均为阴性,对FAdV-4的最低核酸检出量为12.9 pg。该方法可用于禽腺病毒4型的临床检测与流行病学调查。
译  名:
Development and Application of PCR Assay for Detection on Fowl Adenovirus 4
作  者:
Yuan Wanzhe;Zhan Shan;Chen Ping;Zhang Chao;Jing Mei;Cui Yuan;Sun Jiguo;Liu Juxiang;College of Animal Medicine,Agriculture University of Hebei;Hebei Engineering and Technology Research Center of Veterinary Biotechnology;Shijiazhuang Animal biological Products Supply Station;
关键词:
fowl adenovirus 4(FAd V-4);;PCR;;detection
摘  要:
According to Hexon gene sequence of fowl adenovirus 4(FAdV-4)published in Gen Bank,one pair of specific primers was designed for amplifying the specific fragments.PCR assay was developed for detection of FAdV-4.The sensitivity and specificity detections suggested that the PCR assay was so sensitive that it could detect the 12.9 pg level.Specific PCR products of 954 bp could be amplified by the PCR assay.But the detection results for avian influenza virus,newcastle disease virus,infectious bronchitis virus and fowl adenovirus 11 by the PCR assays were all negative.The PCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of FAdV-4 infection.

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