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Position: Home > Articles > In Vitro Pollen Germination Study on A Rare and Endangered Specie: Pinusdabeshanensis Cheng et Law Chinese Agricultural Science Bulletin 2014,30 (13) 15-19

珍稀濒危物种大别山五针松花粉离体萌发的研究

作者：

韩建伟;王恩茂;张智勇;李颖岳

单位：

安徽省岳西县林业总场;北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室

关键词：

大别山五针松;花粉;萌发率;花粉管生长

摘要：

为了研究大别山五针松濒危的原因,并为人工杂交育种和保护该物种提供理论依据,试验采用花粉离体萌发法,设置正交试验,研究了蔗糖、硼、钙对其萌发率及花粉管生长的影响。结果表明:蔗糖是影响大别山五针松花粉萌发和花粉管生长的关键因子,其次是硼酸,氯化钙对其影响最小;蔗糖的添加,明显促进花粉萌发,但高浓度蔗糖花粉管的生长有很大的抑制作用;适当添加硼酸可以促进花粉萌发,但不利于花粉管的生长;氯化钙对花粉萌发影响最小,不同浓度的氯化钙均可以获得较高的萌发率。综合萌发率、花粉管生长,大别山五针松花粉萌发的最适培养基为:4%蔗糖+0.05%硼酸+0.025%氯化钙。

译名：

In Vitro Pollen Germination Study on A Rare and Endangered Specie: Pinusdabeshanensis Cheng et Law

作者：

Han Jianwei;Wang Enmao;Zhang Zhiyong;Li Yingyue;Key National Engineering Laboratory for Tree Breeding/ Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University;Forestry Center in Yuexi County;

关键词：

Pinusdabeshanensis Cheng et Law;;pollen;;germination rate;;pollen tube growth

摘要：

In order to detect the reasons why the Pinusdabeshanensis Cheng et Law is endangered and providethe evidence for the breeding and conservation. The author applied designed the orthogonal test to identify theeffect of sucrose, boron, calcium on germination rate and pollen tube length using in vitro pollen germinationmethods. The results showed that: sucrose was the key factor in its germination and growth followed by boricacid, and calcium chloride played the least role. The ratio of pollen germination could be significantlyincreased by adding sucrose while it also could be limited when the sucrose was in the high level. Appropriateaddition of boric acid could promote pollen germination rather than pollen tube growth. Calcium chloride hadlimited effect on pollen germination and it seemed that germination rate was not sensitive to the level ofcalcium chloride. Integrating pollen germination rate and tube growth, the optimum medium forPinusdabeshanensis Cheng et Law pollen culture should be 4% sucrose+0.05% boric acid+0.025% calciumchloride.

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珍稀濒危物种大别山五针松花粉离体萌发的研究

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