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当前位置: 首页 > 文章 > 有机磷/拟除虫菊酯混剂筛选棉铃虫品系的交互抗性和生化机理(英文) 棉花学报 2008,20 (4) 249-255
Position: Home > Articles > Cross Resistance Patterns and Biochemical Mechanisms in a Chinese Helicoverpa armigera Strain Selected with an Organophosphate/Pyrethroid Mixture Cotton Science 2008,20 (4) 249-255

有机磷/拟除虫菊酯混剂筛选棉铃虫品系的交互抗性和生化机理(英文)

作  者：
杨亦桦;陈松;吴益东
单  位：
农业部病虫监测与治理重点开放实验室南京农业大学植物保护学院
关键词：
棉铃虫;交互抗性;抗性机理;酯酶;多功能氧化酶
摘  要：
用辛硫磷/氟氯氰菊酯(25:1,有效成分)混剂对棉铃虫田间种群(HN)进行33代筛选获得抗性品系(HN-R)。筛选获得的HN-R抗性品系对辛硫磷和氟氯氰菊酯的抗性分别达17.4倍和144.7倍。HN-R品系对两种拟除虫菊酯产生了高水平交互抗性(对氯氰菊酯和氰戊菊酯分别为86.2和23.4倍),对久效磷产生了中等水平交互抗性(5.2倍),对甲基对硫磷(1.6倍)、丙溴磷(1.8倍)和灭多威(2.4倍)产生了低水平交互抗性。酯酶抑制剂DEF能有效抑制HN-R品系对辛硫磷的抗性(增效比为15.3倍),而多功能氧化酶抑制剂PBO则对辛硫磷没有任何增效作用;PBO则使HN-R品系对3种拟除虫菊酯的抗性显著降低(对氟氯氰菊酯、溴氰菊酯和氰戊菊酯的增效比分别为21.9、19.1和21.4倍)。DEF和PBO都能使HN-R对久效磷的抗性下降一半(增效比分别为2.2和2.5倍)。HN-R抗性品系多功能氧化酶活性(以pNA为底物)和酯酶活性(以a-NA为底物)分别为HN品系的6.5倍和1.6倍。因此,在HN-R抗性品系中至少存在2种代谢抗性机理,即增强的酯酶解毒代谢(对辛硫磷、久效磷)和多功能氧化酶介导的氧化解毒代谢(对3种拟除虫菊酯、久效磷)。本研究表明,在田间使用有机磷/拟除虫菊酯混剂可能同时选择出多种代谢抗性机理,从而导致广谱的交互抗性。
译  名：
Cross Resistance Patterns and Biochemical Mechanisms in a Chinese Helicoverpa armigera Strain Selected with an Organophosphate/Pyrethroid Mixture
作  者：
YANG Yi-hua,CHEN Song,WU Yi-dong(Key Laboratory of Monitoring and Management of Plant Diseases and Insects,Ministry of Agriculture,College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China)
关键词：
Helicoverpa armigera Hübner;cross-resistance;resistance mechanism;esterase;mixed function oxidase
摘  要：
Selection of a field strain of Helicoverpa armigera(HN) was undertaken by treatment with a mixture of an organophosphate(phoxim) + a pyrethroid(cyhalothrin)(25:1,a.i.) for 33 generations.The resistance to phoxim of the selected strain(HN-R) increased to 17.4-fold and the resistance to cyhalothrin increased to 144.7-fold.High level cross-resistance to two pyrethroids was detected(86.2-fold to deltamethrin and 23.4-fold to fenvalerate) in the HN-R strain.In addition,the HN-R strain also possessed a moderate level of cross-resistance to monocrotophos(5.2-fold),a low level cross-resistance to methyl parathion(1.6-fold),profenofos(1.8-fold),and methomyl(2.4-fold).The esterase inhibitor S,S,S-tributylphosphorotrithioate(DEF) was very effective in reducing phoxim resistance of the HN-R strain(with a synergism ratio of 15.3-fold) but the mixed function oxidase(MFO) inhibitory synergist piperonyl butoxide(PBO) had no synergism to it at all.In contrast,PBO dramatically removed most of resistance to three pyrethroids with synergism ratios of 21.9-fold to cyhalothrin,19.1-fold to deltamethrin,and 21.4-fold to fenvalerate,but DEF had no synergism to them.Both DEF and PBO can remove about half of the resistance to monocrotophos(with synergism ratio of 2.2-and 2.5-fold,respectively).Compared to the reference HN strain,the MFO activity of the resistant HN-R strain to p-nitroanisole(pNA) was 6.5-fold and total esterase activity to 1-naphthyl acetate(a-NA) was 1.6-fold.At least two metabolic resistance mechanisms are involved in this resistant HN-R strain: esteratic detoxification(to phoxim and monocrotophos) and oxidative metabolism by mixed function oxidases(to pyrethroids and monocrotophos).The results suggest the use of pyrethroid/organophosphate mixtures in the field is likely to lead the simultaneous selection of multiple metabolic mechanisms of resistance,and thus a broad cross-resistance spectrum.

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