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当前位置: 首页 > 文章 > 与甘蓝型油菜隐性核不育rf基因连锁标记的开发 中国油料作物学报 2011,33 (3) 216-220
Position: Home > Articles > Development of molecular markers linked to rf gene of recessive genic male sterility in rapeseed(Brassica napus L.) Chinese Journal of Oil Crop Sciences 2011,33 (3) 216-220

与甘蓝型油菜隐性核不育rf基因连锁标记的开发

作  者：
杨立勇;刘灶长;周熙荣;王伟荣;李延莉;蒋美艳;孙超才
单  位：
上海市农业生物基因中心;上海市农业科学院
关键词：
甘蓝型油菜;隐性核不育;隐性抑制基因;临保系;AFLP;SCAR
摘  要：
甘蓝型油菜不育系20118A为三隐性上位互作遗传模式,其不育性受两对重叠隐性不育基因(a和b)与一对隐性上位抑制基因(rf)互作控制。rf基因纯合时对a和b基因起抑制作用,使油菜育性恢复,可用于核不育三系法育种。为缩短临保系的育种周期,本文利用一个20118A与其临保系M-6029的BC1分离群体获得了7个与rf连锁的AFLP(扩增片段长度多态性)标记,构建了局部连锁图,图距在3cM～17cM。其中距rf最近(3cM)的标记为EA10MC03。用引物对F3和R3成功地将EA10MC03转化成SCAR(序列特异性扩增区)标记,能区分杂合型Rfrf单株和纯合型RfRf单株,为分子标记辅助选择隐性核不育临保系奠定了基础。
译  名：
Development of molecular markers linked to rf gene of recessive genic male sterility in rapeseed(Brassica napus L.)
作  者：
YANG Li-yong1,LIU Zao-chang2,ZHOU Xi-rong1,WANG Wei-rong1, LI Yan-li1,JIANG Mei-yan1,SUN Chao-cai1(1.Shanghai Academy of Agricultural Sciences,Shanghai 201106,China; 2.Shanghai Agro-biological Gene Central,Shanghai 201106,China)
关键词：
Brassica napus L;Recessive genic male sterility(RGMS);Recessive inhibitor gene(rf);Temporary maintainer;AFLP;SCAR
摘  要：
A recessive genic male sterility(RGMS) line 20118A was controlled by 2 recessive sterile genes(a and b) interacting with an inhibitor gene rf.Gene rf restored the fertility and was epistatic to a and b genes.The object of this study was to develop molecular markers linked to rf gene for marker-assisted selection of temporary maintainer line.By using a BC1 population from 20118A×M-6029,7 AFLP(amplified fragment length polymorphisms) markers were obtained and made into a linkage map of rf gene.The genetic distances ranged from 3cM to 17cM.Among them,EA10MC03 marker was 3cM to rf gene.It was transferred into SCAR(sequence-characterized amplified region) marker using primer pair F3 and R3,which could identify the heterozygous individuals(Rfrf genotype) from homozygous ones(RfRf genotype).The markers showed their ability in developing temporary maintainer lines in the future.

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