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Position: Home > Articles > Study on Tissue Culture of Calycanthus chinensis Journal of Zhejiang Forestry Science and Technology 2006,26 (5) 28-30

夏蜡梅组织培养试验初报

作  者:
邵果园;蔡荣荣;王力超;梁国鲁
单  位:
浙江林学院
关键词:
夏蜡梅;组织培养;增殖;生根
摘  要:
以夏蜡梅茎段为外植体,进行夏蜡梅组培试验,结果表明:外植体茎段的最佳消毒方式是先用70%酒精消毒10-15 s,再用0.1%HgCl溶液浸泡3-5 min;继代增殖培养的最佳培养基为MS+6-BA2.0mg/L+ NAA 0.1mg/L+蔗糖30 g/L,增殖系数4.03,茎芽生长粗壮;最佳生根培养基为1/2MS+IBA0.4mg/L。
译  名:
Study on Tissue Culture of Calycanthus chinensis
作  者:
SHAO Guo-yuan, CAI Rong-rong, WANG Li-chao, LIANG Guo-lu (Zhejiang Forestry College Forestry and Biotechnology Faculty, Lin'an 311300,China; Southwest University College of Horticulture and Landscape, Chongqing 400716, China)
关键词:
Calycanthus chinensis; tissue culture; multiplication; rooting
摘  要:
Experiment on tissue culture of Calycanthus chinensis by using stems as explant showed that the optimal sterilizing method was dipping the stems in 70% alcohol for 10 - 15 seconds and 0.1% Hgcl for 3 - 4 minutes. The optimum medium for multiplication was MS + 6-BA2.0mg/L + NAA0.1mg/L + sugar 30 g/L, and the rate of regeneration reached 4.03 with the IBA concentration of 0.4 mg/L in 1/2MS.

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