关键词：
骨修复材料;钛;丝素蛋白;磷酸钙;仿生矿化;生物相容性
摘 要：
将丝素蛋白涂覆到骨修复材料钛金属的表面,调控钛基体上磷酸钙晶体的仿生矿化。采用场发射扫描电子显微镜(FESEM)观察形貌、X射线衍射谱(XRD)、傅里叶变换红外光谱(FTIR)对矿化不同时间丝素蛋白涂覆钛片表面的磷酸钙晶体生长状况进行表征,分析丝素蛋白调控磷酸钙仿生矿化的机理;将MG-63细胞与涂覆丝素蛋白并矿化的钛片共培养,通过MTT法检验细胞的增殖并测定细胞碱性磷酸酶的活性,评价其生物相容性。结果显示:丝素蛋白膜可以诱导磷酸钙在钛片表面沉积,加快磷酸钙晶体的形成速度;晶体呈圆边片层状,并且沿002晶面(c轴)取向生长,羟基磷灰石特征峰增多,说明丝素蛋白可以诱导磷酸钙晶体向羟基磷灰石相生长;形成的晶体涂层有利于MG-63细胞的增殖与分化,具有良好的生物相容性。
译 名：
Effect of Silk Fibroin Membrane on Regulating Crystal Growth of Calcium Phosphate on Titanium Surface
作 者：
ZHANG Jin-Li;ZHANG Qi;XIE Chun-Gang;REN Xiao-Yuan;KONG Xiang-Dong;Institute of Biological Materials,College of Life Science,Zhejiang Sci-Tech University;Qixin College,Zhejiang Sci-Tech University;
关键词：
Bone repair material;;Titanium;;Silk fibroin;;Calcium phosphate;;Biomimetic mineralization;;Biocompatibility
摘 要：
Silk fibroin was coated onto the surface of titanium used as bone repair material to regulate biomimetic mineralization of calcium phosphate on it.Growth status of calcium phosphoate on titanium surface after mineralization for different time was characterized and analyzed by field emission scanning electron microscopy(FESEM),X-ray diffraction(XRD),and Fourier transform infrared spectroscopy(FTIR) to explore the mechanism of silk fibroin in regulating biomimetic mineralization of calcium phosphate.Furthermore,MG-63 cells were co-cultured with the silk fibroin coated and mineralized titanium to evaluate its biocompatibility by using MTT method to measure cell proliferation and determine cellular alkaline phosphatase activity.The results showed that silk fibroin film could induce calcium phosphate to deposit on the titanium surface and accelerate the rate of crystal formation.The crystal morphology was lamellar with a circular edge.Besides,the crystal on the silk fibroin-coated titanium preferred to grow along 002 crystal plane(c axis),and the characteristic peaks of hydroxyapatite increased,which indicated that silk fibroin could make calcium phosphate to have a trend of phase transition from octacalcium phosphate to hydroxyapatite.Furthermore,the obtained crystal was conductive to the proliferation and differentiation of MG-63 cells,showing excellent biocompatibility.
